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UCOE® Expression Technology - Mammalian Expression

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UCOE® Expression Technology

Ubiquitous Chromatin Opening Element (UCOE®) technology gives major improvements in gene expression in stably transfected mammalian cells through effects on the structure of chromatin. UCOE® technology prevents transgene silencing and gives consistent, stable and high-level gene expression irrespective of the chromosomal integration site. UCOE® technology expression elements are small DNA elements (isolated from around house-keeping genes, which need to be active most of the time) which create a transcriptionally active open chromatin environment around an integrated transgene, maximizing its potential to be transcribed into protein irrespective of the position of the transgene in the chromosome.

UCOE References

Webinar:

Expediting and Increasing Recombinant Protein Production in Biomanufacturing Applications
using the UCOE® Gene Expression Platform

Webinar Presenters: 
Michael N Antoniou, MA (Oxon), Ph.D., King’s College London
Joe Orlando, Ph.D., Merck
  • Understand the molecular mechanisms hindering recombinant protein expression in mammalian cell lines
  • Review current strategies to overcome obstacles in recombinant protein expression
  • Review the suitability of the UCOE® technology to expedite the generation of cell lines for biomanufacturing
  • Discuss the seamless integration of the UCOE® technology into established biomanufacturing processes
Watch Now


Merck:/Freestyle/BI-Bioscience/Genomic-Analysis/UCOE_FY_EM_eLit-1-cover.jpg

Benefits

  • Rapid: Grams of protein in < 30 days
  • Boosts: Expression levels up to 20-fold higher than conventional vectors without UCOE® technology
  • Stable: Pools of cells stable > 130 generations
  • High yield: gram quantities of protein
  • Easy to use: Small DNA elements, capable of making two protein chains per plasmid, e.g., antibodies
  • Versatile: Antibodies, EPO, GPCRs, Enzymes, Cytokines, etc., expressed
  • Compatible: Enhances industry standard platforms, e.g., CHO cells, CMV promoters, etc.
  • Serum-free: Protein-free media used throughout
  • Scaleable: Up to 100 L in shake flasks and fermentors
  • Reproducible: Stably-transfected pools can be frozen, providing working stocks for further production without the need for re-transfection
  • Transferable: UCOE®-containing vectors are also suitable for large-scale manufacture
If you are interested in obtaining a license for the UCOE® expression vector technology, please contact Marie Azzaria at Marie.Azzaria@emdmillipore.com  or James Hoberg at James.Hoberg@emdmillipore.com for further information.

Cat No.
Description
Size
5.04865.0001 UCOE® Expression Vector – Mouse 3.2 kb Puro Set 1 SET
5.04866.0001 UCOE® Expression Vector – Mouse 3.2 kb Hygro Set 1 SET
5.04867.0001 UCOE® Expression Vector – Human 4 kb Puro Set 1 SET
UC0E01 UCOE® Single Expression Puromycin Vector Set 1 SET
UC0E02 UCOE® Single Expression Hygromycin Vector Set 1 SET
UC0E03 UCOE® Dual Expression Puromycin Vector Set 1 SET
UC0E04 UCOE® Dual Expression Hygromycin Vector Set 1 SET
UC0E05 UCOE® Intermediate Vector Set 1 SET
 
To order product please call customer service at 1-800-645-5476. 

UCOE Mammalian Protein Expression

Merck:/Freestyle/BI-Bioscience/Genomic-Analysis/UCOEMammalianProteinExpression.jpgLarge scale manufacture of a recombinant GFP fusion protein using conventional mammalian expression system (Left) vs. UCOE mammalian expression system (Right). UCOE technology gives major improvements in gene expression in stably transfected mammalian cells through effects on the structure of chromatin. UCOE technology prevents transgene silencing and gives consistent, stable and high-level gene expression irrespective of the chromosomal integration site yielding 20-fold higher protein expression levels than conventional vectors.