protein+sample+preparation Search Results

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

GEL-FREE SAMPLE PREPARATION FOR THE NANOSCALE LC-MS/MS ANALYSIS AND IDENTIFICATION OF LOW-NANOGRAM PROTEIN SAMPLES
Protein identification at the low nanogram level could in principle be obtained by most nanoscale LC-MS/MS systems. Nevertheless, the complex sample preparation procedures generally required in biological applications, and the consequent high risk of sample losses, very often hamper practical achievement of such low levels. In f...
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C5737
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ZipTip^® Pipette Tips
Measurement of human surfactant protein-B turnover in vivo from tracheal aspirates using targeted proteomics.
We describe a method to measure protein synthesis and catabolism in humans without prior purification and use the method to measure the turnover of surfactant protein-B (SP-B). SP-B, a lung-specific, hydrophobic protein essential for fetal-neonatal respiratory transition, is present in only picomolar quantities in tracheal aspir...
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Reference

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Multiple
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Identification and quantification of Gi-type GTP-binding proteins that copurify with a pituitary somatostatin receptor
Somatostatin (SRIF) receptors of GH4C1 cells occupied with biotinyl-NH-[Leu8,D-Trp22,Tyr25] somatostatin28 (bio-S28) have been affinity purified over streptavidin affinity columns (Eppler, C. M., Zysk, J. R., Corbett, M., and Shieh, H.-M. (1992) J. Biol. Chem. 267, 15603-15612). This procedure results in the copurification of a...
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Reference

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Multiple
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Multiple
Spinning-disk confocal microscopy of yeast.
Spinning-disk confocal microscopy is an imaging technique that combines the out-of-focus light rejection of confocal microscopy with the high sensitivity of wide-field microscopy. Because of its unique features, it is well suited to high-resolution imaging of yeast and other small cells. Elimination of out-of-focus light signifi...
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Reference

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protein+sample+preparation

GEL-FREE SAMPLE PREPARATION FOR THE NANOSCALE LC-MS/MS ANALYSIS AND IDENTIFICATION OF LOW-NANOGRAM PROTEIN SAMPLES

Measurement of human surfactant protein-B turnover in vivo from tracheal aspirates using targeted proteomics.

Identification and quantification of Gi-type GTP-binding proteins that copurify with a pituitary somatostatin receptor

Spinning-disk confocal microscopy of yeast.