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70060 pBACgus4x-1 DNA

pBACgus4x-1 DNA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70060
  
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      Overview

      Description
      Overview

      This product has been discontinued.





      pBACgus4x-1 is a baculovirus transfer plasmid designed for cloning and co-expression of up to four target genes or multiple copies of the same gene in insect cells. The plasmid contains two polh promoters and two p10 promoters, each of which is upstream of unique cloning sites for sequential insertion of target genes. The homologous promoters are in opposite orientations to minimize recombination. The plasmid also carries the gus gene encoding b-glucuronidase under control of the late basic protein promoter (P6.9), which serves as a reporter to verify recombinant viruses by staining with X-gluc. The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the promoters. Optional expression of a C-terminal His•Tag® fusion sequence is possible from the polh right-hand promoter by allowing read-through of inserts in the proper reading frame. Unique restriction sites are indicated on the circle map. The cloning/expression region of the coding strand transcribed from the clockwise promoters is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to this strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer).
      Catalogue Number70060
      Brand Family Novagen®
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Global Trade Item Number
      Catalogue Number GTIN
      70060 0