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484235 p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem

p-Nitroblue Tetrazolium Chloride, CAS 298-83-9, is a NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: NBT, Nitro BT

Primary Target: NOS
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484235
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
298-83-9C₄₀H₃₀Cl₂N₁₀O₆

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      484235-250MG
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          Description
          OverviewNADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).
          Catalogue Number484235
          Brand Family Calbiochem®
          SynonymsNBT, Nitro BT
          References
          ReferencesPistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
          De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis. 16, 29.
          Libon, C., et al. 1993. J. Leukoc. Biol. 53, 93.
          Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
          Nichols, K., et al. 1992. Neuroscience 51, 791.
          Nichols, K., et al. 1992. Neuroscience 51, 791.
          Product Information
          CAS number298-83-9
          FormYellow crystalline solid
          Hill FormulaC₄₀H₃₀Cl₂N₁₀O₆
          Chemical formulaC₄₀H₃₀Cl₂N₁₀O₆
          Hygroscopic Hygroscopic
          Structure formula ImageStructure formula Image
          Quality LevelMQ100
          Applications
          Applicationp-Nitroblue Tetrazolium Chloride, CAS 298-83-9, is a NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions.
          Biological Information
          Primary TargetNOS
          Primary Target IC<sub>50</sub>3-4 µM
          Purity≥98% by TLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSXF8045000
          Safety Information
          R PhraseR: 20/21/22-68

          Harmful by inhalation, in contact with skin and if swallowed.
          Possible risks of irreversible effects.
          S PhraseS: 22-26-36/37

          Do not breathe dust.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing and gloves.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Harmful
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          484235-1GM 07790788054755
          484235-250MG 04055977200690

          Documentation

          p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem Certificates of Analysis

          TitleLot Number
          484235

          References

          Reference overview
          Pistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
          De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis. 16, 29.
          Libon, C., et al. 1993. J. Leukoc. Biol. 53, 93.
          Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
          Nichols, K., et al. 1992. Neuroscience 51, 791.
          Nichols, K., et al. 1992. Neuroscience 51, 791.

          Brochure

          Title
          Pathways and Biomarkers of Oxidative Stress
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-August-2008 RFH
          SynonymsNBT, Nitro BT
          DescriptionNADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).
          FormYellow crystalline solid
          CAS number298-83-9
          RTECSXF8045000
          Chemical formulaC₄₀H₃₀Cl₂N₁₀O₆
          Structure formulaStructure formula
          Purity≥98% by TLC
          SolubilityMeOH or H₂O
          Storage Protect from light
          +2°C to +8°C
          Hygroscopic
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.
          Toxicity Harmful
          ReferencesPistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
          De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis. 16, 29.
          Libon, C., et al. 1993. J. Leukoc. Biol. 53, 93.
          Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
          Nichols, K., et al. 1992. Neuroscience 51, 791.
          Nichols, K., et al. 1992. Neuroscience 51, 791.