Millipore Sigma Vibrant Logo

109215 Ziehl-Neelsen carbol-fuchsin solution

for microscopy

Ziehl-Neelsen carbol-fuchsin solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Carbol fuchsin solution

View Products on Sigmaaldrich.com
109215
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
1092150100
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Glass bottle 100 ml
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      1092150500
      Retrieving availability...
      —
      Limited Availability Limited Availability
      In Stock 
      Discontinued
      Limited Quantities Available
      Availability to be confirmed
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          		 Plastic bottle 500 ml
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          —
          Request Pricing
          		 —
          1092150507
          Retrieving availability...
          —
          Limited Availability Limited Availability
          In Stock 
          Discontinued
          Limited Quantities Available
          Availability to be confirmed
            Remaining : Will advise
              Remaining : Will advise
              Will advise
              Contact Customer Service
              Contact Customer Service

              		 Plastic bottle 500 ml
              Retrieving price...
              Price could not be retrieved
              Minimum Quantity is a multiple of
              Maximum Quantity is
              Upon Order Completion More Information
              You Saved ()
               
              —
              Request Pricing
              		 —
              1092152500
              Retrieving availability...
              —
              Limited Availability Limited Availability
              In Stock 
              Discontinued
              Limited Quantities Available
              Availability to be confirmed
                Remaining : Will advise
                  Remaining : Will advise
                  Will advise
                  Contact Customer Service
                  Contact Customer Service

                  		 Plastic bottle 2.5 l
                  Retrieving price...
                  Price could not be retrieved
                  Minimum Quantity is a multiple of
                  Maximum Quantity is
                  Upon Order Completion More Information
                  You Saved ()
                   
                  —
                  Request Pricing
                  		 —
                  Description
                  Catalogue Number109215
                  SynonymsCarbol fuchsin solution
                  DescriptionZiehl-Neelsen carbol-fuchsin solution
                  OverviewZiehl-Neelsen carbolfuchsin solution - for microscopy, is a ready-to use solution used in the Ziehl-Neelsen hot staining for the microscopic investigation of acid-fast bacteria(AFB). The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Cat. no. 100327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use Löfföer's methylene blue solution(Cat. no. 101287) is used for counterstaining. Those three solutions are each 500 ml and are contained in the AFB-color modified staining kit (Cat. No.100497).
                  The 500 ml bottle of Ziehl-Neelsen carbolfuchsin solution provides approx. 250 stainings. Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. Ziehl-Neelsen carbolfuchsin solution - for microscopy is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
                  References
                  Product Information
                  HS Code3212 90 90
                  Quality LevelMQ400
                  Applications
                  Applicationfor microscopy
                  Biological Information
                  Physicochemical Information
                  Density0.99 g/cm3 (20 °C)
                  Flash point47 °C
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Hazard Pictogram(s) Hazard Pictogram(s)
                  Hazard Pictogram(s)
                  Hazard Pictogram(s)
                  Hazard Pictogram(s)
                  Hazard Statement(s)H226: Flammable liquid and vapour.
                  H314: Causes severe skin burns and eye damage.
                  H341: Suspected of causing genetic defects.
                  H410: Very toxic to aquatic life with long lasting effects.
                  Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
                  P273: Avoid release to the environment.
                  P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
                  P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
                  P304 + P340 + P310: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor.
                  P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
                  Signal WordDanger
                  Storage class3 Flammable liquids
                  WGKWGK 3 highly hazardous to water
                  Disposal9
                  Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
                  Safety Information
                  Categories of dangerflammable, harmful, corrosive, mutagenic
                  Product Usage Statements
                  Storage and Shipping Information
                  StorageStore at +15°C to +25°C.
                  Packaging Information
                  Transport Information
                  Declaration (railroad and road) ADR, RIDUN 1992 , 3 (6.1), III
                  Declaration (transport by air) IATA-DGRUN 1992 , 3 (6.1), III
                  Declaration (transport by sea) IMDG-CodeUN 1992 , 3 (6.1), III
                  Supplemental Information
                  Specifications
                  Global Trade Item Number
                  Catalogue Number GTIN
                  1092150100 04022536112008
                  1092150500 04022536112015
                  1092150507 04054839116780
                  1092152500 04022536112039

                  Documentation

                  Ziehl-Neelsen carbol-fuchsin solution SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Ziehl-Neelsen carbol-fuchsin solution Certificates of Analysis

                  Product Number PackagingSpecificationLot Number
                  1092150100 Glass bottlePDF Specification Document
                  1092150500 Plastic bottlePDF Specification Document
                  1092150507 Plastic bottlePDF Specification Document
                  1092152500 Plastic bottlePDF Specification Document

                  User Guides

                  Title
                  User Guide (IFU) - 109215

                  Related Products & Applications

                  Alternative Products

                  Catalogue Number Description  
                  100497 AFB-Color modified Show Pricing & Availability

                  Accessories (Primary)

                  Catalogue Number Description  
                  100327 Hydrochloric acid in ethanol Show Pricing & Availability
                  101287 Löffler's methylene blue solution Show Pricing & Availability
                  116450 AFB-Color staining kit Show Pricing & Availability

                  Accessories (Secondary)

                  Catalogue Number Description  
                  100497 AFB-Color modified Show Pricing & Availability

                  Related Products By: Application Facete

                  Bacteria Staining

                  Categories

                  IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Auxiliaries for Bacteriology
                  IVD/OEM Materials & Reagents
                  IVD/OEM Materials & Reagents > Microscopy > Bacteriology > General Bacteriological Staining Solutions
                  IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Acid-Fast Bacteria Staining