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69212 S•Tag™ Rapid Assay Kit

Sensitive colorimetric quantification of S•Tag fusion proteins

S•Tag™ Rapid Assay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69212
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      With the S•Tag™ Rapid Assay, a sample is added to a buffer containing the ribonuclease substrate poly(C). The reaction is started by adding purified S-protein. After a 5-minute incubation, the reaction is stopped with trichloroacetic acid. The resulting precipitate is removed by centrifugation. Activity is measured by reading the absorbance of the supernatant at 280 nm, which increases as the poly(C) is broken down into acid-soluble nucleotides by the enzyme. By comparing the results with a known S-peptide standard included in the kit, the molar concentration of target protein in the sample can be determined. As little as 20 fmol of target protein can be detected in a 5-minute incubation with the S•Tag Rapid Assay. Samples can be crude extracts prepared with BugBuster®, PopCulture®, CytoBuster™, or Reportasol™ reagents; SDS; subcellular fractions; translation reactions; or purified proteins. In most applications, there is negligible interference from endogenous RNases.

      The S•Tag Rapid Assay Kit includes a set of matched reagents designed to perform 100 standard assays.
      Catalogue Number69212
      Brand Family Novagen®
      References
      ReferencesRaines, R.T., et al. 2000. Meth. Enzymol. 326, 362.
      McCormick, M. and Mierendorf, R. 1994 inNovations 1, 4–7.
      Kim, J.-S. and Raines, R.T. 1993 Protein Sci. 2, 348–356.
      Richards, F.M. and Wyckoff, H.W. 1971 in The Enzymes, Vol. IV (Boyer, P.D., Ed.), p. 647–806, Academic Press, New York.
      Product Information
      •1 mlS•Tag Grade S-protein
      •4 ml10X S•Tag Assay Buffer
      •50 µlS•Tag Standard (0.05 pmole/µl)
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      69212 0

      Documentation

      S•Tag™ Rapid Assay Kit Certificates of Analysis

      TitleLot Number
      69212

      References

      Reference overview
      Raines, R.T., et al. 2000. Meth. Enzymol. 326, 362.
      McCormick, M. and Mierendorf, R. 1994 inNovations 1, 4–7.
      Kim, J.-S. and Raines, R.T. 1993 Protein Sci. 2, 348–356.
      Richards, F.M. and Wyckoff, H.W. 1971 in The Enzymes, Vol. IV (Boyer, P.D., Ed.), p. 647–806, Academic Press, New York.

      Citations

      Title
    • Jiping Xiao, et al. (2006) Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis. Journal of Biological Chemistry 281, 15182-15193.
    • Marina V. Backer, et al. (2003) Engineering S-protein fragments of bovine ribonuclease A for targeted drug delivery. Protein Expression and Purification 26, 455–461.
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      User Protocols

      Title
      TB082 S•Tag™ System