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03-0202-00 SMC® Human NF-L High Sensitivity Immunoassay Kit

The SMC® Human NF-L High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure Neurofilament Light Chain P

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03-0202-00
96 well assay  
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      Overview

      Description
      Catalogue Number03-0202-00
      Trade Name
      • SMC®
      DescriptionSMC® Human NF-L High Sensitivity Immunoassay Kit
      OverviewThe SMC® Human NF-L High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
      Background InformationThe SMC® Human NF-L High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure Neurofilament Light Chain Protein (NF-L) in Human Serum, Plasma and Cerebrospinal Fluid (CSF) samples. A capture antibody specific for Human NF-L has been pre-coated onto paramagnetic microparticles (beads). The user pipettes beads, standards, and samples into uncoated microplate wells. During incubation, the NF-L present in the sample binds to the capture antibody on the coated beads. Unbound molecules are washed away during the subsequent wash steps. Fluor-labeled detection antibody is added to each well and incubated. This detection antibody recognizes and binds to NF-L that has been captured onto the beads, thus completing the immunosandwich. Elution buffer is then added and incubated. The elution buffer dissociates the bound protein sandwich from the beads surface releasing the labeled antibodies. These antibodies are separated during transfer to a 384-well read plate. The plate is loaded into the SMCxPRO® System where the labeled molecules are detected and counted. The number of fluor-labeled detection antibodies counted is directly proportional to the amount of NF-L present in the sample when captured. The amount of NF-L in unknown samples is interpolated from a standard curve.
      Product Information
      Components
      • Assay Buffer
      • NF-L Coated Beads
      • Standard Diluent
      • NF-L Detection Antibody
      • NF-L Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      Precision, %
      • The assay variations of SMC® Human NF-L Immunoassay kits were studied using five normal plasma samples run in triplicate by 3 different operators on 3 different days.
      • Mean intra-assay variation was 10.2%.
      • Mean inter-assay variation was 9.6%.
      Quality LevelMQ200
      Applications
      ApplicationThe SMC® Human NF-L High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure Neurofilament Light Chain Protein (NF-L) in Human Serum, Plasma and Cerebrospinal Fluid (CSF) samples.
      Key Applications
      • Single Molecule Counting (SMC®)
      Application NotesNeurofilament Light Chain (NF-L) is a low molecular weight member of a family of proteins involved in axonal development and function. NF-L accumulation is viewed as a telltale sign of axonal damage in the brain, and is a key marker in a wide range of neurodegenerative diseases, especially as a blood-based biomarker. We are proud to aid these efforts by offering the new SMC® Human NF-L High Sensitivity Immunoassay Kit. By coupling this assay with the powerful state-of-the-art SMCxPRO® detection system, researchers can accurately assess NF-L concentrations even in low abundance in human serum, plasma, and CSF samples, enabling studies that examine the relevance of this key analyte from health to disease.

      This new streamline kit has the following features:

      • Improved options for sample prep and incubation
      • Lyophilization protein standard for uniform shipping and storage condition
      • Increased reagent volumes to accommodate automation
      • Rigorously developed to achieve accurate and reproducible performance on the state-of-the-art SMCxPRO® ultrasensitive immunoassay system
      Biological Information
      Cross ReactivityCross-reactivity to the following analytes were tested with the following results:
      NF-M: Not detected
      NF-H: Not detected
      pTau (pT181): Not detected
      Total Tau: Not detected
      GFAP: Not detected
      Accuracy
      • Minimum of 4 serum samples spiked with average recovery of 93% with a range of 77-105%
      • Minimum of 4 plasma samples spiked with average recovery of 94% with a range of 78-104%
      • Minimum of 5 CSF samples spiked with average recovery of 123% with a range of 99-165%
      Physicochemical Information
      Sensitivity
      • Lower Limit of Quantification: 0.87 pg/mL
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Packaging Information
      Material Size96 well assay
      Global Trade Item Number
      Catalogue Number GTIN
      03-0202-00 04065266090406