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03-116 RIPAb+™ STAU1 (Staufen 1) - RIP Validated Antibody and Primer Set

This RIPAb+ STAU1 (Staufen 1) -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting, Immunoprecipitation, RNA Binding Protein Immunoprecipitation (RIP) Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors, RNA Metabolism & Binding Proteins
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03-116
10 assays  10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context).
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      Overview

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      Key Spec Table

      Species ReactivityKey Applications
      HWB, IP, RIP
      Description
      Catalogue Number03-116
      Trade Name
      • RIPAb+
      • Upstate
      DescriptionRIPAb+™ STAU1 (Staufen 1) - RIP Validated Antibody and Primer Set
      OverviewRIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
      STAU1 (Staufen 1) is a RNA-binding protein implicated in the transport and/or localization of mRNA via the microtubule network to the rough endoplasmic reticulum.
      Product Information
      FormatPurified
      Control
      • Includes negative control normal rabbit IgG antibody and control primers specific for the human ARF1 mRNA.
      PresentationAnti-STAU1 (Staufen 1) (Rabbit Polyclonal),. Lyophilized powder. Add 50 μL of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
      Normal Rabbit IgG. One vial containing 125 µg of purified rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
      RIP Primers, ARF1.
      One vial containing 75 μL of 5 μM of each primer specific for human ARF1 mRNA.
      Store at -20°C.
      FOR: GAC CAC GAT CCT CTA CAA GC
      REV: TCC CAC ACA GTG AAG CTG ATG
      Quality LevelMQ100
      Applications
      ApplicationThis RIPAb+ STAU1 (Staufen 1) -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • RNA Binding Protein Immunoprecipitation (RIP)
      Application NotesImmunoprecipitation from RIP lysate:
      Representative lot data.
      RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or 5 µg of Anti-STAU1 (Staufen 1) antibody (Cat. # CS204405). Ten percent of the precipitated proteins (lane 1: rabbit IgG, lane 2: STAU1) were resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-STAU1 (Staufen 1) antibody (Cat. # CS204405, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232) .
      Arrow indicates STAU1. (Figure 2).
      Western Blot Analysis:
      Representative lot data.
      HepG2 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-STAU1 antibody (Cat. # CS204405, 1:1000 dilution). Proteins were visualized using a Goat Anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system.
      Arrow indicates STAU1 (~63 kDa). (Figure 3).
      Note: Uncharacterized bands appear at ~22 kDa and ~53 kDa in
      Biological Information
      ImmunogenSynthetic peptide from amino acids 2-51 of human STAU 1.
      Epitopea.a. 2-51
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with human. Other species have not yet been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Molecular Weight~63 kDa observed. Uncharacterized bands appear at ~22 kDa and ~53 kDa in some lysates.
      Product Usage Statements
      Quality AssuranceRNA Binding Protein Immunoprecipitation:
      RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal Rabbit IgGor 5 µg of Anti-STAU1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
      Successful immunoprecipitation of STAU1-associated RNA was verified by qPCR using RIP Primers ARF1, (Figure 1).
      Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size10 assays
      Material Package10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context).
      Global Trade Item Number
      Catalogue Number GTIN
      03-116 04053252756528