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539486 Protein Kinase A, Catalytic Subunit, Bovine Heart

Convenient kit for site-specific ³²P-labeling of target proteins

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: cAMP-Dependent Protein Kinase Catalytic Subunit

539486
  
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      Overview

      Description
      OverviewThe PKAce™ Kit is designed for in vitro labeling of proteins in a simple reaction with 32P-γ-ATP and purified Protein Kinase A (PKA). Proteins can be labeled to at least 105 cpm/µg in 10 minutes, and can be used directly as sensitive and specific probes for protein:protein interactions (1). The PKAce Kit includes a highly purified preparation of bovine heart PKA, optimized reaction buffer, and PKA Control Protein for verification of kit performance.
      Mediates the majority of the actions of cAMP. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Stimulates Ca2+-dependent exocytosis in bovine chromaffin cells. Suitable for studies of PKA phosphorylation in vitro. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.
      Catalogue Number539486
      Brand Family Calbiochem®
      SynonymscAMP-Dependent Protein Kinase Catalytic Subunit
      References
      ReferencesRazani, B., et al. 1999. J. Biol. Chem. 274, 26353. Gross, R.A., et al. 1994. Mol. Endocrinol. 8, 970. Bosma, M.M., et al. 1993. J. Neurosci. 13, 5242. Brindle, P., et al. 1993. Nature 364, 821. Hajnoczky, G., et al. 1993. Biochem. J. 293, 413. Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10135. Wang, L-Y., et al. 1993. Science 259, 1173.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to histone per min at 30°C.
      EC number2.7.1.37
      FormLiquid
      FormulationIn 100 mM NaCl, 30 mM DTT, 20 mM MES, 100 µM EDTA, 50% glycerol, pH 6.5.
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥750 units/µg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage DEEP FREEZE (-70°C)
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      539486 0