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This PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb is validated for use in Immunoblotting for the detection of VEGFR2 (pTyr1054/1059).
More>>This PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb is validated for use in Immunoblotting for the detection of VEGFR2 (pTyr1054/1059). Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Recognizes the ~200 kDa VEGFR-2 protein phosphorylated at Tyr1054/1059 in porcine pancreatic cells transfected with a CSF-1/mouse VEGFR-2 chimeric receptor.
Detection of mouse VEGFR2, phosphorylated at Tyr1054/1059, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with CSF-1/mouse VEGFR2 chimeric receptor left unstimulated (lanes 1,2) or stimulated with CSF-1 (lanes 3-8). Primary antibodies: PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb (Cat. No. PS1013) (0.5 µg/ml) pre-incubated without peptide (lanes 2,4,5), with non-phosphopeptide corresponding to the immunogen (lane 6), a generic phosphotyrosine-containing peptide (lane 7), or the phosphopeptide immunogen (lane 8) or pan anti-VEGFR2 antibody (lanes 1,3). Detection: chemiluminescence.
References
References
Meyer, R.D., et al. 2002. J. Biol. Chem.277, 27081. Zeng, H., et al. 2001. J. Biol. Chem.276, 32714. Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci.902, 201. Rahimi, N., et al. 1999. Mol. Biol. Cell10, 3401.
Product Information
Form
Liquid
Formulation
In Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (± 0.1).
Positive control
Porcine aortic endothelial (PAE) cells transfected with a CSF-1/mouse VEGFR2 chimeric receptor
The immunogen sequence is 100% conserved in human and rat VEGFR2, but reactivity has not been tested. The corresponding phosphorylation site in VEGFR3 is 100% conserved, but cross-reactivity has not been tested. When used at a 1:1000 dilution there is sufficient antibody for 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1054/1059 phosphorylation sites of mouse and human VEGFR-2
Host
Rabbit
Isotype
IgG
Species Reactivity
Mouse
Antibody Type
Polyclonal Antibody
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Irritant
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number
GTIN
PS1013-10T
04055977227086
Documentation
PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb Certificates of Analysis
Title
Lot Number
PS1013
References
Reference overview
Meyer, R.D., et al. 2002. J. Biol. Chem.277, 27081. Zeng, H., et al. 2001. J. Biol. Chem.276, 32714. Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci.902, 201. Rahimi, N., et al. 1999. Mol. Biol. Cell10, 3401.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of mouse VEGFR2, phosphorylated at Tyr1054/1059, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with CSF-1/mouse VEGFR2 chimeric receptor left unstimulated (lanes 1,2) or stimulated with CSF-1 (lanes 3-8). Primary antibodies: PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb (Cat. No. PS1013) (0.5 µg/ml) pre-incubated without peptide (lanes 2,4,5), with non-phosphopeptide corresponding to the immunogen (lane 6), a generic phosphotyrosine-containing peptide (lane 7), or the phosphopeptide immunogen (lane 8) or pan anti-VEGFR2 antibody (lanes 1,3). Detection: chemiluminescence.
Description
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~200 kDa VEGFR2 protein phosphorylated at Tyr1054/1059.
Background
Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) is receptor tyrosine kinase that serves as a key mediator in angiogenesis and plays an essential role in embryonic development, wound healing, cell proliferation, migration, and differentiation. It contains seven immunoglobulin-like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 (FMS-like tyrosine kinase 1) and Flt-4 (FMS-like tyrosine kinase 4). Upon binding to its ligand, VEGF, VEGFR2 undergoes ligand-induced dimerization, which activates its intrinsic tyrosine kinase activity resulting in auto- and trans-phosphorylation on specific tyrosine residues in the cytoplasmic domain. These tyrosine residues, when phosphorylated, are involved as docking sites to recruit molecules containing Src homology 2 (SH2) or phosphotyrosine-binding domains and to convey signals to downstream effectors. Phosphorylation of VEGFR2 at Tyr1054 and Tyr1059 within the activation loop is required for required for activation of VEGFR2 and enhances its intrinsic tyrosine kinase activity. Phosphorylation at Tyr1059 is also responsible for VEGF-induced intracellular Ca2+ mobilization.
Host
Rabbit
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1054/1059 phosphorylation sites of mouse and human VEGFR-2
Isotype
IgG
Species
mouse
Positive control
Porcine aortic endothelial (PAE) cells transfected with a CSF-1/mouse VEGFR2 chimeric receptor
Form
Liquid
Formulation
In Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (± 0.1).
Preservative
≤0.1% sodium azide
Comments
The immunogen sequence is 100% conserved in human and rat VEGFR2, but reactivity has not been tested. The corresponding phosphorylation site in VEGFR3 is 100% conserved, but cross-reactivity has not been tested. When used at a 1:1000 dilution there is sufficient antibody for 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Irritant
References
Meyer, R.D., et al. 2002. J. Biol. Chem.277, 27081. Zeng, H., et al. 2001. J. Biol. Chem.276, 32714. Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci.902, 201. Rahimi, N., et al. 1999. Mol. Biol. Cell10, 3401.
