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525286 PhosphoDetect™ Anti-Phosphothreonine Mouse mAb (14B3)

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525286
  
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      Overview

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes phosphothreonine-containing proteins. Supplied with a positive control.
      Catalogue Number525286
      Brand Family Calbiochem®
      Application Data
      Detection of phosphothreonine-containing proteins by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibody: Anti-Phosphothreonine Mouse mAb (14B3) (Cat. No. 525286) (1 µg/ml). Detection: chemiluminescence. *Positive control supplied with the antibody. Available separately: Anti-Phosphothreonine (1E11) (mouse) (Cat. No. 525287), and Phosphothreonine Detection Kit (Cat. No. 525288)
      Product Information
      FormLyophilized
      Formulation50 µg antibody lyophilized from 2X PBS, PEG, sucrose and 150 µl control lysate lyophilized from 2x PBS, PEG, sucrose, 10 mM NaF.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Immunoprecipitation (1-10 µg/10⁶ vandate-treated A431 cells)
      Application CommentsFor immunoblotting with the positive control, use 5 µl (chemiluminescence) to 20 µl (colorimetric) per mini-gel lane. Use BSA for membrane blocking and antibody dilutions, do not use milk/casein. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena mixture of synthetic phosphopeptides containing phosphothreonine, conjugated to KLH
      Clone14B3
      HostMouse
      IsotypeIgG₁
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute antibody with 1 ml H₂O (15 min, RT). Following reconstitution, aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 year at -70°C or for up to 3 months at 4°C. Thaw aliquots at 37°C. Solubilize rabbit muscle positive control with 200 µl H₂O. Add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
      Global Trade Item Number
      Catalogue Number GTIN
      525286 0