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14-791M PI 3-Kinase (p110α(E542K)/p85α) murine, 250 µg

Complex of N-terminal 6His-tagged recombinant murine p110α full length, containing the mutation E542K & untagged, recombinant, murine p85α full length.

14-791M
250 µg  Also available in 10 μg size (cat#14-791) and in bulk (cat# 14-791-K).
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      Overview

      Description
      Catalogue Number14-791M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPI 3-Kinase (p110α(E542K)/p85α) murine, 250 µg
      OverviewComplex of N-terminal 6His-tagged recombinant murine p110α full length, containing the mutation E542K, and untagged, recombinant, murine p85α full length.
      Background InformationThe E542K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation. (Kang S. et al., PNAS, (2005);102: 802-807 and Zhao J.J. et al., PNAS, (2005);102:18443-18448).
      Product Information
      Presentation50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
      Quality LevelMQ300
      Applications
      ApplicationComplex of N-terminal 6His-tagged recombinant murine p110α full length, containing the mutation E542K & untagged, recombinant, murine p85α full length.
      Biological Information
      SourceCo-expressed by baculovirus in Sf21 insect cells.
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Gene Symbol
      • PIK3CA
      • PI3K
      • PK3CA
      Protein TargetPI3Kα/p85α
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyPI3K/PI4K
      UniProt Number
      Molecular Weightp110α(E542K) MW = 129 kDa, p85α MW = 84 kDa.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-791) and in bulk (cat# 14-791-K).
      Global Trade Item Number
      Catalogue Number GTIN
      14-791M 04053252322556