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MAB3242-KC Mouse Anti-Smoothelin, clone R4A (1mg) KC

Detect Smoothelin using this Anti-Smoothelin Antibody, clone R4A validated for use in WB, IC, IH(P).

Mouse Anti-Smoothelin, clone R4A (1mg) KC MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
MAB3242-KC
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      Description
      Catalogue NumberMAB3242-KC
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionMouse Anti-Smoothelin, clone R4A (1mg) KC
      OverviewThis product is intended for use by IVD manufacturers.
      Contact customer service for assistance: oem.reagents@emdmillipore.com
      Alternate Names
      • Anti-Smoothelin
      Background InformationSmoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cell with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin or vimentin. Smoothelin colocalizes with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 50 kDa isoform specific for visceral SMC, and an isoform with a molecular weight of approximately 100 kDa in vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.MAB3242 is useful to discriminate fully differentiated, contractile SMCs from proliferative SMCs. The distribution of smoothelin-positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions.
      Product Information
      FormatPurified
      PresentationLiquid in buffer (PBS) with 0.1% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationDetect Smoothelin using this Anti-Smoothelin Antibody, clone R4A validated for use in WB, IC, IH(P).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot

      Immunocytochemistry

      Immunohistochemistry on both frozen and paraffin embedded tissue sections. For paraffin sections, high temperature antigen retrieval in 10 mM citrate buffer (pH 6.0) is highly recommended (van der Loop, 1997).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenCytoskeletal extract of chicken gizzard.
      CloneR4A
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB3242 reacts with smoothelin, a cytoskeletal protein exclusively found in smooth muscle cells. Reacts with the 59 kDa and 100 kDa protein corresponding to smoothelin by Western blot.
      IsotypeIgG1
      Species Reactivity
      • Canine
      • Chicken
      • Human
      • Hamster
      • Monkey
      • Pig
      • Feline
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in three transcript variants.
      Gene Symbol
      • SMTN
      • smoothelin
      • Smoothelin.
      • FLJ38597
      • SMSMO
      • FLJ35365
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P53814 # Structural protein (cytoskeletal).
      SIZE: 917 amino acids; 99516 Da
      SUBCELLULAR LOCATION: Cytoplasm. Note=Exhibits a filamentous organization.
      TISSUE SPECIFICITY: Smooth muscle; contractile or vascular (for the long form).
      SIMILARITY: SwissProt: P53814 ## Contains 1 CH (calponin-homology) domain.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material SizeBulk packaging. Please inquire.
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3242-KC 04053252683107