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FCMAB101A4 Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated

Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated detects level of BrdU & has been published & validated for use in FC & IC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: BrdU
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FCMAB101A4
100 tests  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue NumberFCMAB101A4
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated
      Alternate Names
      • BrdU
      Background InformationBromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. This antibody does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody's special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}.
      Product Information
      FormatAlexaFluor®488
      Control
      • After incorporation of BrdU, all DNA containing species
      PresentationPresentation needs to say: Purified mouse monoclonal IgG2a supernatant conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/transmission.
      Quality LevelMQ100
      Applications
      ApplicationMilli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated detects level of BrdU & has been published & validated for use in FC & IC.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      Biological Information
      ImmunogenBromodeoxyuridine
      CloneBu-1
      HostMouse
      SpecificitySpecifically binds to bromodeoxyuridine (crossreacts with iodouridine 10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Species Reactivity NoteTested on Human. Predicted to react with all species.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • BrdU
      Purification MethodProtein G Purified
      Molecular Weightnone given
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry with Jurkat Cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C from date of receipt. Protect from light.
      Packaging Information
      Material Size100 tests
      Global Trade Item Number
      Catalogue Number GTIN
      FCMAB101A4 04053252742194