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14-548 MSK1 Protein, active, 10 µg

Active, N-terminal His6-tagged recombinant human MSK1 residues 2-end, for use in Kinase Assays.

MSK1 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-548
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-548M when ordering the 250 µg size.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-548
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMSK1 Protein, active, 10 µg
      OverviewN-terminal His6-tagged recombinant human MSK1 residues 2-end
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal His6-tagged recombinant human MSK1 residues 2-end, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Gene Symbol
      • RPS6KA5
      • RSKL
      • RLPK
      • MGC1911
      • MSK1
      • MSPK1
      Protein TargetMSK1
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O75582 # Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
      COFACTOR: Magnesium.
      SIZE: 802 amino acids; 89865 Da
      SUBUNIT: Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Predominantly nuclear. Partially cytoplasmic.
      TISSUE SPECIFICITY: Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
      PTM: Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C- terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.
      SIMILARITY: SwissProt: O75582 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 2 protein kinase domains.
      MISCELLANEOUS: Enzyme activity requires the presence of both kinase domains.
      Molecular Weight94kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of Crosstide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -20°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-548M when ordering the 250 µg size.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-548 04053252619953

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-548M MSK1 Protein, active, 250 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases