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QIA129 InnoCyte™ Cell Invasion Assay Kit (24-well)

InnoCyte™ Cell Invasion Assay Kit (24-well) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
QIA129
  
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      Overview

      Key Spec Table

      Detection Methods
      Fluorescence
      Description
      Overview

      This product has been discontinued.



      We are offering QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric (Cat. No. ECM554) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






      A convenient and useful assay for the quantitative measurement of cell invasion. Contains 12 cell culture inserts with an 8 µm polycarbonate membrane. This membrane is coated with a thin layer of a biological matrix. This layer prevents noninvasive cells from going through the membrane. Cells are quantified utilizing a highly sensitive fluorescent dye (Excitation: ~485 nm; Emission: ~520 nm).
      Catalogue NumberQIA129
      Brand Family Calbiochem®
      Materials Required but Not Delivered• Pipettors with disposable tips
      • 96-well plate or strips in black or white color
      • Fluorescence plate reader
      • Tissue culture equipment
      • Forceps
      • Serum-free tissue culture medium
      • Tissue culture medium with 10% FCS
      References
      ReferencesMontell, D.J. 2003. Nat. Rev. Mol. Cell Biol. 4, 13.
      Egblad, M., et al. 2002. Nat. Rev. Cancer 2, 161.
      Hood, J.D., et al. 2002. Nat. Rev. Cancer 2, 91.
      Chatterjee, N., et al. 2001. Environ. Pathol. Toxicol. Oncol. 20, 211.
      Koul, D., et al. 2001. Oncogene 20, 6669.
      Kleinman, H.K., et al. In Molecular and Cellular Aspects of Basement Membranes, Rohrbach, D.H. and Timpl, R., eds. Academic Press, 1993, pp. 309.
      Repesh, L.A. 1989. Invasion Metastasis 9, 192.
      Albini, A., et al. 1987. Cancer Res. 47, 3229.
      Terranova, V.P., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 465.
      Liotta, L.A., 1984. Am. J. Pathol. 117, 339.
      Product Information
      Detection methodFluorescence
      Form12 Tests
      Format24-well plate
      Kit contains24-Well Plate containing 12 cell culture inserts coated with Basement Membrane Extract, Cell Detachment Solution, Cell Staining Solution, Cell Staining Diluent, and a user protocol.
      Quality LevelMQ100
      Biological Information
      Assay time24-48 h
      Sample TypeCulture cells
      Product Usage Statements
      Intended useThe Calbiochem® InnoCyte™ Cell Invasion Assay is a quantitative assay for the study of tumor cell invasion in vitro. The assay is useful for discriminating between invasive and non-invasive tumor cell lines and for screening anti-cancer drugs that affect cancer cell invasion. Cell invasion is quantified by labeling the invaded cells with calcein-AM.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage Multiple storage requirements
      Storage ConditionsUpon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.
      Do not freeze Ok to freeze
      Supplemental Information
      Kit contains24-Well Plate containing 12 cell culture inserts coated with Basement Membrane Extract, Cell Detachment Solution, Cell Staining Solution, Cell Staining Diluent, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      QIA129 0