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218709 Casein Kinase II, α2, Active, Human, Recombinant, E. coli

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218709
  
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      Overview

      Description
      Overview

      This product has been discontinued.



      Recombinant, human casein kinase II, α2, (CKII, α2) fused to maltose binding protein (MBP) at the N-terminus and expressed in E. coli. CKII, α2 is a catalytic subunit of the serine/threonine protein kinase CKII. It is suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.

      Catalogue Number218709
      Brand Family Calbiochem®
      SynonymsCK2α2, CSNK2A2
      Application Data
      Increasing concentrations of Casein Kinase II, α2 were assayed according to the protocol outlined above.
      References
      ReferencesAntonelli, M., et al. 1997. Eur. J. Biochem. 241, 272.
      Xu, X., et al. 1997. Genomics 48, 79.
      Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to RRRADDSDDDDD peptide substrate per min at 30°C.
      FormLiquid
      FormulationIn 50 mM Tris-HCl, 500 mM NaCl, 10 mM DTT, pH 8.5.
      Quality LevelMQ100
      Biological Information
      Purity≥ 90% by SDS PAGE
      Specific Activity≥841.819 Units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      218709 0