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382065 Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - CAS 23491-52-3 - Calbiochem

Cell-permeable, adenine-thymine-specific fluorescent stain.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342

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382065
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Overview

Key Spec Table

CAS #Empirical Formula
23491-52-3C₂₇H₂₈N₆O · 3HCl

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Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
382065-100MG
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      Description
      OverviewCell-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei for fluorescence microscopy and flow cytometry applications. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H 33342 and the related compound H 33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10-fold greater cell-permeability than H 33258.
      Catalogue Number382065
      Brand Family Calbiochem®
      Synonyms2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342
      References
      ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
      Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
      Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
      Belloc, F., et al. 1994. Cytometry 17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
      Hinkley, R.E., et al. 1986. Dev. Biol. 118, 148.
      Sandhu, L.C., et al. 1985. Cytometry 6, 191.
      Frankfurt, O.S. 1983. Cytometry 4, 216.
      Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 363.
      Visser, J.W.M. 1980. Flow Cytometry IV, 86.
      Product Information
      CAS number23491-52-3
      ATP CompetitiveN
      FormYellow solid
      Hill FormulaC₂₇H₂₈N₆O · 3HCl
      Chemical formulaC₂₇H₂₈N₆O · 3HCl
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Biological Information
      Primary TargetAdenine-thymine-specific fluorescent stain
      Purity≥95% by TLC
      Physicochemical Information
      Cell permeableY
      Emission max.
      Excitation max.
      Safety Information according to GHS
      RTECSDT4200000
      Safety Information
      R PhraseR: 23/24/25-36/37/38

      Toxic by inhalation, in contact with skin and if swallowed.
      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36/37/39-45

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Toxic & Carcinogenic / Teratogenic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions (1 mg/ml) are stable for up to 2 weeks at 4°C. Precipitates in phosphate buffer solutions.
      Global Trade Item Number
      Catalogue Number GTIN
      382065-100MG 04055977213089