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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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196418
MilliporeBAPTA, Tetrasodium Salt
Ca2+ chelator exhibiting a 105-fold greater affinity for Ca2+ .
More>>Ca2+ chelator exhibiting a 105-fold greater affinity for Ca2+ . Less<<
BAPTA, Tetrasodium Salt MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Ca2+ chelator exhibiting a 105-fold greater affinity for Ca2+ (Kd = 110 nM) than for Mg2+. After BAPTA complexes with Ca2+,the UV spectrum shifts from 254 to 279 nm at pH >6.0. Useful for spectrophotometric monitoring of extracellular Ca2+ levels, especially transient phenomena, because of its insensitivity to pH. Useful for the measurement and control of Ca2+ levels in eggs, chromaffin cells, muscle cells, retinal cells, and pancreatic cells. In conjunction with certain formazan dyes, BAPTA can be used in a colorimetric assay for Mg2+.Has neuroprotective effects in vitro against excitotoxic damage and spontaneous recurrent seizures, such as epilepsy.
DeLorenzo, R.J., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 14482. Li, L., et al. 1995. J. Cell Physiol. 163, 105. Tymianski, M., et al. 1994. J. Neurophysiol. 72, 1973.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Irritant
Storage
-20°C
Protect from Light
Protect from light
Hygroscopic
Hygroscopic
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot, purge with inert gas and freeze (-20°C). To increase stability in solution, store at a high pH (>10). Stock solutions are stable for up to 1 month at -20°C.
DeLorenzo, R.J., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 14482. Li, L., et al. 1995. J. Cell Physiol. 163, 105. Tymianski, M., et al. 1994. J. Neurophysiol. 72, 1973.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Ca2+-chelator exhibiting a 105-fold greater affinity for Ca2+ (110 nM) than for Mg2+. After BAPTA complexes with Ca2+, the UV spectrum shifts from 254 to 279 nm at pH >6.0. Useful for spectrophotometric monitoring of extracellular Ca2+ levels, especially transient phenomena, because of its insensitivity to pH. Has been used for the measurement and control of Ca2+ levels in eggs, chromaffin cells, muscle cells, retinal cells, and pancreatic cells. In conjunction with certain formazan dyes, BAPTA can be used in a colorimetric assay for Mg2+. Has neuroprotective effects in vitro against excitotoxic damage and spontaneous recurrent seizures, such as epilepsy.
Form
White to off-white solid
CAS number
126824-24-6
Chemical formula
C₂₂H₂₀N₂O₁₀ · 4Na
Structure formula
Solubility
H₂O (10 mg/ml)
Storage
Protect from light -20°C Hygroscopic
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot, purge with inert gas and freeze (-20°C). To increase stability in solution, store at a high pH (>10). Stock solutions are stable for up to 1 month at -20°C.
Toxicity
Irritant
References
DeLorenzo, R.J., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 14482. Li, L., et al. 1995. J. Cell Physiol. 163, 105. Tymianski, M., et al. 1994. J. Neurophysiol. 72, 1973.
