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ABE1057 Anti-acetyl Histone H3 Antibody (Lys 64)

Anti-acetyl Histone H3 Antibody is an antibody against acetyl Histone H3 for use in Western Blotting, Immunocytochemistry, Dot Blot.

Anti-acetyl Histone H3 Antibody (Lys 64) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ UniProt Number: P68431
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ABE1057
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, DBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1057
      DescriptionAnti-acetyl Histone H3 Antibody (Lys 64)
      Alternate Names
      • Histone H3.1
      • Histone H3/a
      • Histone H3/b
      • Histone H3/c
      • Histone H3/d
      • Histone H3/f
      • Histone H3/h
      • Histone H3/I
      • Histone H3/j
      • Histone H3/k
      • Histone H3/l
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci. Histone H3 is reported to be acetylated at Lys64 (H3K64ac) by p300 co-activator. In addition, H3K64ac is found enriched at the transcriptional start sites of active genes, consistent with a transcriptional activation modification opposite of its repressive counterpart H3K64me3 (PMID 24668167). The target specificity of ABE1057 is confirmed with peptide-based epitope analysis by dot blot, using Cat. No. 16-667, AbSurance Histone H3 Antibody Specificity Array, and Cat. No. 16-665, AbSurance Histone H2A, H2B, H4 Antibody Specificity Array. Sequence surrounding the target acetylation site is conserved among human Histone H3 subtypes H3.1, H3.2, H3.3, and H3.3C/H3.5 (UniProt P68431, Q71DI3, P84243, and Q6NXT2, respectively).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-acetyl Histone H3 Antibody is an antibody against acetyl Histone H3 for use in Western Blotting, Immunocytochemistry, Dot Blot.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Dot Blot
      Application NotesImmunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected acetyl Histone H3 (Lys 64) in HeLa, A431, and NIH/3T3 cells.
      Dot Blot Specificity Analysis: Histone peptides with various modifications were probed with acetyl Histone H3 (Lys 64) using a 1:250 dilution.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human acetyl Histone H3 (Lys 64).
      EpitopeAcetylated Lys 64
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      Modifications
      • Acetylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in sodium butyrate treated HeLa cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected acetyl Histone H3 (Lys 64) in 10 µg of sodium butyrate treated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1057 04055977163957