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AB5062P Anti-Vesicular GABA Transporter (VGAT) Antibody

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AB5062P
50 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, H, MkELISA, IF, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5062P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Vesicular GABA Transporter (VGAT) Antibody
      Alternate Names
      • Vesicular Inhibitory Amino Acid Transporter
      Background InformationVesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter (1). VGAT functions in the uptake of GABA and glycine into synaptic vesicles. Since the protein is bound to synaptic vesicles, VGAT antibodies are excellent markers of presynaptic GABAergic and glycinergic neurons.
      Product Information
      FormatAffinity Purified
      Control
      • Brain tissue, rat retina lysate
      PresentationPurified rabbit polyclonal in buffer containing PBS with 0.1% BSA.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Vesicular GABA Transporter (VGAT) Antibody is validated for use in ELISA, IF, IH for the detection of Vesicular GABA Transporter (VGAT).
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesImmunohistochemistry/Immunofluorescence:
      2-20 µg/mL of a previous lot was used on paraformaldehyde fixed sections.

      ELISA:
      1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenA 17 amino acid peptide sequence near the carboxy terminal region of rat VGAT (McIntire, S., 1997).
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificitySpecific for Vesicular GABA Transporter (VGAT). Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter. The immunogen peptide shows no significant homology with other known proteins.
      Species Reactivity
      • Rat
      • Mouse
      • Human
      • Monkey
      Species Reactivity NoteRat. Antibody cross-reactivity with various species is not known. The immunogen sequence is 100% conserved in mouse, human and monkey.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.
      Gene Symbol
      • SLC32A1
      • VIAAT
      • VGAT
      • bA122O1.1
      • hVIAAT
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9H598 # Involved in the uptake of GABA and glycine into the synaptic vesicles.
      SIZE: 525 amino acids; 57415 Da
      SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane protein (By similarity).
      TISSUE SPECIFICITY : Retina. Expressed throughout the horizontal cells or more specifically at the terminals.
      SIMILARITY: SwissProt: Q9H598 ## Belongs to the amino acid/polyamine transporter 2 family.
      Molecular Weight57 kDa
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      Catalogue Number GTIN
      AB5062P 04053252287381