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MABN2547-100UL Anti-VMAT1 Antibody, clone N440 21

Anti-VMAT1, clone N440 21, Cat. No. MABN2547, is a highly specific mouse monoclonal antibody that targets Vesicular Monoamine Transporter 1 and has been tested for use in Western Blotting.

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MABN2547-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2547-100UL
      DescriptionAnti-VMAT1 Antibody, clone N440 21
      Alternate Names
      • Chromaffin granule amine transporter
      • Solute carrier family 18 member 1
      • Vesicular amine transporter 1
      • VAT1
      Background InformationChromaffin granule amine transporter (UniProt: Q8R090; also known as Solute carrier family 18 member 1, Vesicular amine transporter 1, VMAT1, VAT1) is encoded by the Scl19a1 (also known as Vmat1) gene (Gene ID: 110877) in murine species. Vesicular monoamine transporters (VMATs) are involved in presynaptic storage and release of neurotransmitters. They are located in the presynaptic region and are involved in the transport of monoamines into storage vesicle, which are released upon arrival of an action potential. Two structurally related, but pharmacologically distinct, VMATs have been described (VMAT1 and VMAT2) that are encoded by separate genes. VMAT1 is a multi-pass membrane acidic glycoprotein that is found in both large dense-core vesicles and in small synaptic vesicles. It s both C- and N-terminal regions are located in the cytosolic side of the vesicle. VMAT1 is detected in adrenal medulla, and brain (at protein level). In brain, specifically found in the medulla oblongata, pons, prefrontal cortex, striatum, dentate gyrus and hippocampus. VMAT1 is shown to play a key role in survival of hippocampal neurons and may contribute to neurocognitive deficits observed in neuropsychiatric disorders. (Ref.: Multani, PK., et al. (2013). Neurosci. 232; 32-44).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
      Applications
      ApplicationAnti-VMAT1, clone N440 21, Cat. No. MABN2547, is a highly specific mouse monoclonal antibody that targets Vesicular Monoamine Transporter 1 and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenRecombinant fragment corresponding to 93 amino acids from the N-terminal first lumenal region of murine Vesicular amine transporter 1 (VMAT1).
      Epitopeextracellular domain
      CloneN440/21
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone N440/21 specifically detects Vesicular monoamine transporter 1 (VMAT1) and does not show reactivity with VMAT2. It targets an epitope with in 93 amino acids from the N-terminal first lumenal region.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Scl18a1
      • Vmat1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~93 kDa observed; 56.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat cerebellum tissue lysate.

      Western Blotting Analysis: A 1:250 dilution of this antibody detected VMAT1 in rat cerebellum tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2547-100UL 04054839636523

      Documentation