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MAB4308 Anti-VIN-IS-56 Antibody, clone VIN-IS-56

Anti-VIN-IS-56 Antibody, clone VIN-IS-56 detects level of VIN-IS-56 & has been published & validated for use in FC, IC, IH.

Anti-VIN-IS-56 Antibody, clone VIN-IS-56 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Neural Stem Cells Gene Symbol: VIN-IS-56
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MAB4308
100 µg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MaFC, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4308
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-VIN-IS-56 Antibody, clone VIN-IS-56
      Background InformationAlmost all melanomas, astrocytomas,a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen.
      GD3 is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in humans via enhancement of cytotoxic and proliferative response of lymphocytes.
      Anti-GD3 antibodies have been found in association with specific forms of Guillain-Barre syndrome. In vivo studies of isolated anti-GM1 and GD3 antibodies indicate the antibodies can interfere with motor neuron function.
      References
      Product Information
      FormatPurified
      Control
      • Undifferentiated human embryonic stem or embryonic carcinoma cells stain weakly
      PresentationPurified immunoglobulin. Liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-VIN-IS-56 Antibody, clone VIN-IS-56 detects level of VIN-IS-56 & has been published & validated for use in FC, IC, IH.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry & Immunocytochemistry:

      Suggested fixatives: Paraformaldehyde is recommended, NOT methanol or acetone (the glycolipid antigen is extracted by polar lipid solvents).

      Recommended antibody dilution: 10-20 μg/ml

      Dilution Buffer: PBS + 4% FCS

      Expected staining pattern: cell surface staining



      FACS Analysis:

      Suggested dilution/number of cells: 10-20 μg/ml for 1 x 105 cells

      Fixation/Permeabilization used: Live cells or fixed in 4% Paraformaldehyde

      Recommended controls: Undifferentiated human ES or EC cells stain weakly.



      Immunoblotting:

      Not tested



      EIA:

      Not tested



      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman Embryonal carcinoma cells
      CloneVIN-IS-56
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis monoclonal antibody recognizes a ganglioseries glycolipid, gangliosides GD3. Experimental evidence suggests that the expression of this antigen increases following human Embryonal Carcinoma and Embryonic Stem cell differentiation. It is thought that this antibody recognizes cells of the neuroectoderm lineage.
      IsotypeIgM
      Species Reactivity
      • Mammals
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • VIN-IS-56
      Stem Cell Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB4308 04053252576263

      Documentation

      Anti-VIN-IS-56 Antibody, clone VIN-IS-56 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-VIN-IS-56 Antibody, clone VIN-IS-56 Certificates of Analysis

      TitleLot Number
      MOUSE VIN-IS-56 [GD3] PURIFIED MONOCLONAL ANTIBODY - 2443397 2443397
      MOUSE VIN-IS-56 [GD3] - 3709491 3709491
      MOUSE VIN-IS-56 [GD3] -2575779 2575779
      MOUSE VIN-IS-56 [GD3] -2890766 2890766

      References

      Reference overviewPub Med ID
      Surface antigens of human embryonic stem cells: changes upon differentiation in culture
      Draper J.S. et al.
      J. Anat., 200 (Pt 3):249-258 (2002)  2002

      		12033729 12033729
      Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours
      Andrews P.W. et al.
      Int. J. Cancer, 66(6):806-816 (1996)  1996

      		8647654 8647654
      Differentiation antigens of human germ cell tumours: distribution of carbohydrate epitopes on glycolipids and glycoproteins analyzed using PDMP, an inhibitor of glycolipid synthesis
      Fenderson B.A. et al.
      European Urology, 23:30-37 (1993)  1993

      		7682952 7682952
      Different patterns of glycolipid antigens are expressed following differentiation of TERA-2 human embryonal carcinoma cells induced by retinoic acid, hexamethylene bisacetamide (HMBA) or bromodeoxyuridine (BUdR)
      Andrews P.W. et al.
      Differentiation, 43:131-138 (1990)  1990

      		2373286 2373286

      Brochure

      Title
      Human Stem Cell Systems