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ABS2215-100UG Anti-Uromodulin/THP

This rabbit polyclonal Anti-Uromodulin / THP, Cat. No. ABS2215 is tested for use in Western Blotting and Immunohistochemistry (Paraffin), for the detection of Human Uromodulin (Tamm-Horsfall protein,

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ABS2215-100UG
100 μg  
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      Overview

      Description
      Catalogue NumberABS2215-100UG
      DescriptionAnti-Uromodulin/THP
      Alternate Names
      • Tamm-Horsfall urinary glycoprotein
      • THP
      Background InformationUromodulin (UniProt: P07911; also known as Tamm-Horsfall urinary glycoprotein, THP) is encoded by the UMOD gene (Gene ID: 7369) in human. Uromodulin is a homodimeric glycoprotein that is produced in the kidney and can polymerize into long filaments. It is expressed in the tubular cells of the kidney and is most abundant protein in normal urine. It plays a role in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop, where it promotes formation of complex filamentous gel-like structure that may play a role in the water barrier permeability. It also facilitates neutrophil migration across renal epithelia. Uromodulin contains a ZP domain (aa 334-589) that mediates polymerization, leading to the formation of long filaments. The core of the filament consists of stacked ZP domains, which assemble into a helical structure. Each ZP domain consists of an N-terminal (ZP-N; aa 334-429) and C-terminal (ZP-C; aa 465-589) region connected by a flexible linker. The linker allows the ZP domain to wrap around the ZP-C subdomain of the preceding subunit. It is proteolytically cleaved at a conserved C-terminal proteolytic cleavage site to generate the secreted form found in the urine. In the urine, it may contribute to colloid osmotic pressure, retard passage of positively charged electrolytes, and prevent urinary tract infection. It also inhibits the formation of liquid containing supersaturated salts and subsequent formation of salt crystals. Five isoforms of uromodulin have been described that are produced by alternative splicing. (Ref.: Micanovic, R., et al. (2022). Am. J. Physiol. Renal Physiol. 322(4); F403-418; Schmid, M., et al. (2010). Cell Physiol. Biochem. 26(3); 311-318; Santambrogio, S., et al. (2008). Biochem. Biophys. Res. Commun. 370(3); 410-413).
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.02% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis rabbit polyclonal Anti-Uromodulin / THP, Cat. No. ABS2215 is tested for use in Western Blotting and Immunohistochemistry (Paraffin), for the detection of Human Uromodulin (Tamm-Horsfall protein, THP).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested applications

      Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Uromodulin/THP in human kidney tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of Human Uromodulin/THP.
      EpitopeC-terminal
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Uromodulin/THP. It targets an epitope within 22 amino acids from the C-terminal region.
      IsotypeIgGκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • UMOD
      Purification MethodPurified
      UniProt Number
      Molecular Weight~105 kDa observed; 69.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human kidney tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Uromodulin/THP in Human kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2215-100UG 04065268978931