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CP07 Anti-β-Tubulin Mouse mAb (DM1B)

Anti-β-Tubulin Mouse mAb (DM1B) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CP07
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.
      Catalogue NumberCP07
      Brand Family Calbiochem®
      References
      ReferencesBlose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
      Lin, J.J.C. 1984. in Monoclonal antibodies and functional cell lines (Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds.) Plenum Publishing Corp.
      Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
      Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
      Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Positive controlAny cell line
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesImmunoblotting (5 µg/ml)
      Immunofluorescence (5 µg/ml)
      Application CommentsTubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. β-Tubulin has a molecular weight of ~60 kDa (i.e. ~57 kDa in gerbils). Use fresh lysates for immunoblotting, as tubulin may not be detected after a freeze/thaw cycle. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogennative chick brain microtubules
      ImmunogenChicken
      CloneDM1B
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP07 0

      Documentation

      Anti-β-Tubulin Mouse mAb (DM1B) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-β-Tubulin Mouse mAb (DM1B) Certificates of Analysis

      TitleLot Number
      CP07

      References

      Reference overview
      Blose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
      Lin, J.J.C. 1984. in Monoclonal antibodies and functional cell lines (Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds.) Plenum Publishing Corp.
      Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
      Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
      Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-August-2007 RFH
      ApplicationImmunoblotting (5 µg/ml)
      Immunofluorescence (5 µg/ml)
      DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa β-tubulin protein.
      BackgroundCytoskeletal proteins are found in highly organized arrays within the cytoplasm of higher eukaryotic cells. These proteins are intimately involved in functions such as cell and intracellular organelle movement, maintenance of cell shape and endocytosis. The cytoskeleton of most eukaryotic cells is comprised of at least three fibrous systems each with accessory proteins: the microfilaments, composed of actin; the microfibrils, composed of tubulin; and the intermediate filaments composed of vimentin. Monoclonal antibodies have been used to study the components of the cytoskeleton and their intracellular location, changes in the expression of these elements during differentiation, and the function of the cytoskeleton in living cells by microinjection of the antibodies.
      HostMouse
      Immunogen speciesChicken
      Immunogennative chick brain microtubules
      CloneDM1B
      IsotypeIgG₁
      Specieschicken, gerbil, human, mouse, rat
      Positive controlAny cell line
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsTubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. β-Tubulin has a molecular weight of ~60 kDa (i.e. ~57 kDa in gerbils). Use fresh lysates for immunoblotting, as tubulin may not be detected after a freeze/thaw cycle. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesBlose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
      Lin, J.J.C. 1984. in Monoclonal antibodies and functional cell lines (Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds.) Plenum Publishing Corp.
      Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
      Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
      Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.