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GF16 Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2)

This Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of TGF-β3 (Ab-1).

Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Transforming Growth Factor-β3

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes TGF-β2 and TGF-β3 in umbilical cord cells. Does not cross-react with TGF-β1.
      Catalogue NumberGF16
      Brand Family Calbiochem®
      SynonymsAnti-Transforming Growth Factor-β3
      References
      ReferencesBurton, P.B.J., et al. 1993 Int. J. Exp. Path. 74, 87.
      Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol] 327, 145.
      ten Dijke, P., et al. 1990 Mol. Cell. Biol. 10, 4473.
      ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA 85, 4715.
      de Martin, R., et al. 1987 EMBO J. 6, 3677.
      Sporn, M.B., et al. 1987 J. Cell Biol. 105, 1039.
      Derynck, R., et al. 1985 Nature 316, 701.
      Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 6264.
      Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA 77, 5258.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Positive controlRecombinant TGF-β3 protein (Cat. No. PF018) or umbilical cord cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Paraffin Sections (1-10 µg/ml, no pre-treatment required, see comments)
      Application CommentsDoes not cross-react with TGF-β1. For staining paraffin sections, no pre-treatment is required, but the addition of 0.05% Triton® X-100 detergent in the incubation buffer is recommended. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human TGF-β3
      ImmunogenHuman
      Epitopewithin the N-terminal region
      Clone236-5.2
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GF16 0

      Documentation

      Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) Certificates of Analysis

      TitleLot Number
      GF16

      References

      Reference overview
      Burton, P.B.J., et al. 1993 Int. J. Exp. Path. 74, 87.
      Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol] 327, 145.
      ten Dijke, P., et al. 1990 Mol. Cell. Biol. 10, 4473.
      ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA 85, 4715.
      de Martin, R., et al. 1987 EMBO J. 6, 3677.
      Sporn, M.B., et al. 1987 J. Cell Biol. 105, 1039.
      Derynck, R., et al. 1985 Nature 316, 701.
      Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 6264.
      Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA 77, 5258.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-August-2007 RFH
      SynonymsAnti-Transforming Growth Factor-β3
      ApplicationFrozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Paraffin Sections (1-10 µg/ml, no pre-treatment required, see comments)
      DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3x63 Ag 8.653 myeloma cells. Recognizes TGF-β2 and TGF-β3.
      BackgroundTransforming growth factorβs are a family of proteins originally characterized by their ability to act synergistically with EGF or TGFα. TGFβs have been found to produce multiple biological effects including mitogenesis, growth inhibition, chemotaxis and induction or inhibition of differentiation. In their mature form TGFβs are acid- and heat-stable disulfide-linked homodimeric proteins (Mr = ~25 kDa), with each homodimer consisting of 112 amino acid residues. Two homodimeric forms of TGFβ have been identified (TGFβ1 and TGFβ2) which show significant amino acid sequence homology. A new member of the family, TGFβ3 has recently been cloned and characterized. There is greater amino acid homology between TGFβ3 and TGFβ2 as compared with TGFβ1. All TGFβs are released by cells in a biologically latent form. Most cells have high affinity receptors for TGFβ1, β2, and β3.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human TGF-β3
      Epitopewithin the N-terminal region
      Clone236-5.2
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlRecombinant TGF-β3 protein (Cat. No. PF018) or umbilical cord cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsDoes not cross-react with TGF-β1. For staining paraffin sections, no pre-treatment is required, but the addition of 0.05% Triton® X-100 detergent in the incubation buffer is recommended. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBurton, P.B.J., et al. 1993 Int. J. Exp. Path. 74, 87.
      Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol] 327, 145.
      ten Dijke, P., et al. 1990 Mol. Cell. Biol. 10, 4473.
      ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA 85, 4715.
      de Martin, R., et al. 1987 EMBO J. 6, 3677.
      Sporn, M.B., et al. 1987 J. Cell Biol. 105, 1039.
      Derynck, R., et al. 1985 Nature 316, 701.
      Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 6264.
      Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA 77, 5258.