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MABE254 Anti-Protein-arginine deiminase 4 Antibody, clone 10F1

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MABE254
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE254
      DescriptionAnti-Protein-arginine deiminase 4 Antibody, clone 10F1
      Alternate Names
      • HL-60 PAD
      • Protein-arginine deiminase type-4
      • Peptidylarginine deiminase IV
      • Protein-arginine deiminase type IV
      Background InformationProtein-arginine deiminase type-4 (PAD4; formerly PAD5) is one of five PAD (1-4, 6) homologues that belong to the superfamily of multidomain amidinotransferases. The PAD enzymes catalyze deiminating reactions in which guanidinium is hydrolyzed to peptidylcitrulline. PAD4 is a calcium-regulated nuclear-localized enzyme. It modifies arginine residues on histones H2A, H3, and H4 (H2 Arg3; H3 Arg2, H3 Arg8, H3 Arg17, H3 Arg26; and H4 Arg3). Several studies have suggested that PAD4-mediated citrullination has important implications for gene regulation. PAD4 may perform deimination at sites overlapping with arginine methylation, and antagonize the transcription-promoting effects of methylating enzymes such as CARM1 and PRMT1. PAD4 may therefore inhibit gene expression, most notably expression of cell-cycle regulating p53 genes such as p21. Previous studies have suggested that PAD4 may play central role in the development of cancer.
      Product Information
      FormatPurified
      Control
      • DMSO treated and untreated HL-60 cell lysates
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-Protein-arginine deiminase 4 Antibody, clone 10F1 (mouse monoclonal antibody) validated in WB, IP to detect PAD-4 also known as HL-60 PAD, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Protein-arginine deminiase 4 in IP (Prof. K. Mowen, Department of Chemical Physiology, The Scripps Research Institute-California Campus.).

      Chromatin Immunoprecipitation Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated Protein-arginine deminiase 4 in ChIP-Seq (Price, D. H., et al. (2011). Mol Cell. 45(1):38-50.).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Protein-arginine deminiase 4.
      Clone10F1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PADI4
      • PADI5
      • PDI5
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~74 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in DMSO treated and untreated HL-60 cell lysates.

      Western Blot Analysis: 0.25 µg/mL of this antibody detected Protein-arginine deminiase 4 in 10 µg DMSO treated and untreated HL-60 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MABE254 04053252522222