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AB5519 Anti-Prodynorphin Antibody

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AB5519
50 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RIHCGpAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5519
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Prodynorphin Antibody
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified Guinea pig antibody in PBS containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Prodynorphin Antibody is an antibody against Prodynorphin for use in IH.
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:500

      Optimal working dilutions must be determined by the end user.

      APPLICATION NOTES FOR AB5519

      IMMUNOHISTOCHEMISTRY

      Perfusion: 1) Ca2+-free Tyrode's solution followed by 2) paraformaldehyde-picric acid fixative and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

      Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5519. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
      Biological Information
      ImmunogenSynthetic peptide from rat ProDynorphin.
      HostGuinea Pig
      SpecificityProDynorphin, rat.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine.
      Gene Symbol
      • PDYN
      • MGC26418
      • preprodynorphin
      • leumorphin
      • beta-neoendorphin-dynorphin
      • rimorphin
      • prodynorphin
      • PENKB
      • Preprodynorphin
      • leu-enkephalin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01213 # Leumorphin has a typical opiod activity and may have anti-apoptotic effect (By similarity).
      SIZE: 254 amino acids; 28385 Da
      SUBCELLULAR LOCATION: Secreted.
      PTM: The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
      SIMILARITY: SwissProt: P01213 ## Belongs to the opioid neuropeptide precursor family.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Global Trade Item Number
      Catalogue Number GTIN
      AB5519 04053252621635