Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Product Description: Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
Background Information
Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
References
Product Information
Format
Biotin
Control
A431 carcinoma cells. Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting.
Presentation
Biotin-conjugated recombinant mouse IgG2bκ in PBS containing 0.05% sodium azide. Liquid at 2-8°C.
Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate validated for use in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
Phosphotyramine-KLH
Epitope
N/A
Clone
4G10®
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Tyrosine-phosphorylated proteins from all species.
Isotype
IgG2bκ
Species Reactivity
All
Antibody Type
Monoclonal Antibody
Modifications
Phosphorylation
Purification Method
Protein G Purified
Molecular Weight
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Routinely evaluated by western blot to detect tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Western Blot Analysis: 0.5-2 μg/mL of this lot detected tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 9 months at 2-8ºC from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. DO NOT FREEZE!
Study tyrosine phosphorylation using Millipore's specific and sensitive antibodies, including the popular clone 4G10®, and new 4G10 Platinum, available in various conjugations. See below for more. Learn More >>
Phosphotyrosine Antibodies
Millipore’s phosphotyrosine antibodies, including the popular 4G10 clone, demonstrate sensitivity and detect multiple tyrosine phosphorylations on numerous substrates. See below for pTyr antibodies. Learn More >>
