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05-1000 Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)

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05-1000
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtELISA, FC, IF, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1000
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphoserine Antibody, clone 4A4 (mouse IgG1)
      Background InformationThe identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (>95%).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1 in buffer containing PBS with 0.1% sodium azide and 30% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationDetect Phosphoserine using this Anti-Phosphoserine Antibody, clone 4A4 (mouse IgG1) validated for use in ELISA, FC, IF, IH(P) & WB.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunofluorescence

      Flow Cytometry

      Immunohistochemistry (Paraffin)

      ELISA
      Biological Information
      ImmunogenPhosphoserine coupled to KLH.
      Clone4A4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes serine-phosphorylated proteins from all species.
      IsotypeIgG1
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein G-Sepharose™ Chromatography
      Molecular WeightDependent upon the molecular weight of the serine phosphorylated protein being detected.
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.

      Western Blot Analysis:
      0.5–2 μg/mL of this lot detected serinephosphorylated proteins in a lysate from either insulin or Calyculin A/Okadaic-treated human A431 carcinoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      For maximum recovery, centrifuge the original vial prior to cap removal. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      05-1000 04053252586132