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528215
Sigma-AldrichAnti-PAI-1 Mouse mAb (MA-33B8)
This Anti-PAI-1 Mouse mAb (MA-33B8) is validated for use in Immunoblotting, Neutralization Studies for the detection of PAI-1.
More>>This Anti-PAI-1 Mouse mAb (MA-33B8) is validated for use in Immunoblotting, Neutralization Studies for the detection of PAI-1. Less<<
Anti-PAI-1 Mouse mAb (MA-33B8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Recognizes the ~50 kDa PAI-1 protein.
Catalogue Number
528215
Brand Family
Calbiochem®
Synonyms
Anti-Plasminogen Activator Inihibitor-1
References
References
Montes, R., et al. 2000. Thromb. Haemost.84, 65. Verhamme, I., et al. 1999. J. Biol. Chem.274, 17511. Debrock, S., and Declerck, P.J. 1997. Biochim. Biophys. Acta1337, 257.
Product Information
Form
Liquid
Formulation
In 100 mM NaCl, 50 mM sodium phosphate buffer, pH 7.4.
Immunoblotting (10 µg/ml) Neutralization Studies (see application references)
Application Comments
Rapidly inactivates PAI-1 (Plasminogen Activator Inhibitor-1) to the latent form. Like the native latent form, PAI-1 inactivated by antibody was protected from cleavage by elastase. Also shown to reduce renal fibrin deposits in a rabbit model of endotoxemia. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
human tPA-PAI-1 complex
Immunogen
Human
Clone
MA-33B8
Host
Mouse
Purity
≥ 90% by SDS-PAGE
Isotype
IgG
Species Reactivity
Human
Rabbit
Antibody Type
Monoclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
≤ -70°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Anti-PAI-1 Mouse mAb (MA-33B8) Certificates of Analysis
Title
Lot Number
528215
References
Reference overview
Montes, R., et al. 2000. Thromb. Haemost.84, 65. Verhamme, I., et al. 1999. J. Biol. Chem.274, 17511. Debrock, S., and Declerck, P.J. 1997. Biochim. Biophys. Acta1337, 257.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-March-2009 JSW
Synonyms
Anti-Plasminogen Activator Inihibitor-1
Application
Immunoblotting (10 µg/ml) Neutralization Studies (see application references)
Description
Purified mouse monoclonal antibody. Recognizes the ~50 kDa PAI-1 protein.
Host
Mouse
Immunogen species
Human
Immunogen
human tPA-PAI-1 complex
Clone
MA-33B8
Isotype
IgG
Species
human, rabbit
Form
Liquid
Formulation
In 100 mM NaCl, 50 mM sodium phosphate buffer, pH 7.4.
Concentration Label
Please refer to vial label for lot-specific concentration
Purity
≥ 90% by SDS-PAGE
Preservative
None
Comments
Rapidly inactivates PAI-1 (Plasminogen Activator Inhibitor-1) to the latent form. Like the native latent form, PAI-1 inactivated by antibody was protected from cleavage by elastase. Also shown to reduce renal fibrin deposits in a rabbit model of endotoxemia. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw ≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Montes, R., et al. 2000. Thromb. Haemost.84, 65. Verhamme, I., et al. 1999. J. Biol. Chem.274, 17511. Debrock, S., and Declerck, P.J. 1997. Biochim. Biophys. Acta1337, 257.
Application references
Neutralization Studies
Verhamme, I., et al. 1999. J. Biol. Chem.274, 17511.