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MABE1894-100UL Anti-Orc1 Antibody, clone 78-1-172

Anti-Orc1, clone 78-1-172, Cat. No. MABE1894, is a mouse monoclonal antibody that detects Orc1 and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry (Paraffin), and Immunoprecipitation.

MABE1894-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1894-100UL
      DescriptionAnti-Orc1 Antibody, clone 78-1-172
      Alternate Names
      • Origin recognition complex subunit 1
      • Replication control protein 1
      Background InformationOrigin recognition complex subunit 1 (UniProt: Q13415; also known as Replication control protein 1) is encoded by the ORC1 (also known as ORC1L, PARC1) gene (Gene ID: 4998) in human. Orc1 is a component of the ORC complex that binds origins of replication. It is the largest subunit of ORC and its expression is cell-cycle regulated. It begins to accumulate in mid-G1 phase and reaches a peak level at the G1/S boundary. Upon S phase entry, Orc1 is ubiquitinated and targeted for destruction and its levels decline significantly even when levels of other ORC subunits remain unchanged. Its levels increase when cells enter the mitotic phase. DNA-binding of Orc1 is shown to be ATP-dependent. The expression of Orc1 in human cells is reported to be regulated by E2F and the assembly of pre-replication complex at all origins is shown to depend on the E2F/Rb pathway. Orc1 contains a BAH domain (aa 45-171) that mediates its binding to demethylated histone H4 Lysine 20 (H4K20me2), which is enriched at replication origins. Mutations in ORC1 gene are known to cause Meier-Gorlin syndrome that is characterized by severe intrauterine and postnatal growth retardation and poor weight gain. (Ref.: Kara, N., et al. (2015). J. Biol. Chem. 290(19); 12355-12369; Hossain, M., and Stillman, B (2016). eLife. 5; e12785).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Orc1, clone 78-1-172, Cat. No. MABE1894, is a mouse monoclonal antibody that detects Orc1 and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry (Paraffin), and Immunoprecipitation.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Chromatin Immunoprecipitation (ChIP)
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesChromatin Immunoprecipitation Analysis (ChIP): A representative lot detected Orc1 in ChIP applications (Hossain, M., et. al. (2016). Elife. 5:e12785).

      Immunoprecipitation Analysis: A 1:100 dilution from a representative lot immunoprecipitated Orc1 in U20S whole cell lysates. (Courtesy of Michael Tramantano, Ph.D., Cold Spring Harbor Laboratory, New York, USA).

      Immunoprecipitation Analysis: A representative lot detected Orc1 in Immunoprecipitation applications (Kara, N., et. al. (2015). J Biol Chem. 290(19):12355-69).

      Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Orc1 in Saos-2 cells.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 461 amino acids from the C-terminal half of human Origin recognition complex subunit 1 (Orc1).
      EpitopeC-terminal half
      Clone78
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 78-1-172 (also known as clone 78) is a mouse monoclonal antibody that detects human Origin recognition complex subunit 1 (Orc1). It targets an epitope within the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • ORC1
      • ORC1L
      • PARC1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight97.35 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human testis tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Orc1 in human testis tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1894-100UL 04065265324328