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MABN737 Anti-OPA1, clone 1OPA-1A8 Antibody

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MABN737
100 µL  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICC, IPMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN737
      DescriptionAnti-OPA1, clone 1OPA-1A8 Antibody
      Alternate Names
      • Dynamin-like 120 kDa protein, mitochondrial
      • Large GTP-binding protein
      • LargeG
      • Optic atrophy protein 1 homolog
      • Dynamin-like 120 kDa protein, form S1
      Background InformationOPA1 (optic atrophy 1) is a dynamin-related GTPase that plays a role in genome preservation and mitochondrial morphology. Localization of OPA1 is thought to be anchored to the inner membrane of the mitochondria. OPA1 is highly expressed in inner and outer retinal neurons and is important for normal mitochondrial function in those cells. Mutations in OPA1 are the major cause of autosomal dominant optic atrophy. Recent research has found that OPA1 also has the ability to protect neurons from excitotoxic injury and could be a potential clinical target to increase endurance of neurons post brain damage.
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1κ ascites with no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-OPA1 antibody, clone 1OPA-1A8 is validated for use in western blotting, ICC & IP for the detection of OPA1.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected OPA1 in COS cells (Prof. Mustapha Oulad-Abdelghani, IGBMC, France).

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated OPA1 from mouse brain tissue lysate (Akepati, V. R., et al. (2008). J Neurochem. 106(1):372-383.).
      Biological Information
      ImmunogenRecombinant protein corresponding to mouse OPA1.
      Clone10PA-1A8
      HostMouse
      SpecificityThis antibody detects six known isoforms of OPA1 (Akepati, V. R., et al. (2008). J Neurochem. 106(1):372-383.).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Opa1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~90 and ~100 kDa observed. Six isoforms between 80 kDa and 100 kDa are known to exist (Akepati, V. R., et al. (2008). J Neurochem. 106(1):372-383.).
      Product Usage Statements
      Quality AssuranceEvaluated by human brain tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OPA1 in 10 µg of human brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      MABN737 04053252944802