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MAB4081 Anti-MnSOD Antibody, clone MnS-1

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MAB4081
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, RbWB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4081
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MnSOD Antibody, clone MnS-1
      Alternate Names
      • MnSOD
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in PBS.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-MnSOD Antibody, clone MnS-1 is validated for use in WB, IC, IH for the detection of MnSOD.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry on acetone fixed cryostat sections: 0.5-10 μg/mL using an APAAP or PAP procedure. Antibody also reacts with 4% PFA fixed frozen tissue. Typical block contains 1% non-fat dry milk or 3% NGS and 0.2-1.5% triton X-100 {higher triton is only used on thick sections; blocked for 1-3 hours at room temperature. Primary antibody is diluted only in PBS or TBS without excess blocking materials (no triton or milk); typical dilution is 1:200-1:1000 with incubations up to 48 hours at 4C. Washed 3x10' in PBS and detect via DAB. Immunoreactive neurons are characterized by the dark brown, fine granular deposition in soma and proximal dendrites.

      Immunocytochemistry on cell smears: 0.5-10 μg/mL.

      Western blot: MnS1 antibody will recognize at 25kDa band under reducing conditions; this is the monomer of the MnSOD protein.

      Optimal working dilutions must be determined by end user.
      Biological Information
      CloneMnS-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityAntibody reacts with MnSOD
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
      Gene Symbol
      • SOD2
      • MNSOD
      • MnSOD
      • IPO-B
      • Mn-SOD
      • EC 1.15.1.1
      Non-Reactive Species
      • Mouse
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04179 # Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
      COFACTOR: Binds 1 manganese ion per subunit.
      SIZE: 222 amino acids; 24722 Da
      SUBUNIT: Homotetramer.
      SUBCELLULAR LOCATION: Mitochondrion matrix.SIMILARITY:SwissProt: P04179 ## Belongs to the iron/manganese superoxide dismutase family.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsPrior to opening maintain at 2-8°C for up to six month after date of receipt. After opening maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB4081 04053252724176