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MABN2635-25UG Anti-Mitochondrial ferritin/FTMT Antibody, clone C65-2

Anti-Mitochondrial ferritin/FTMT clone C65-2 , Cat. No. MABN2635, is a mouse monoclonal antibody that detects mitochondrial ferritin and is tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

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MABN2635-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2635-25UG
      DescriptionAnti-Mitochondrial ferritin/FTMT Antibody, clone C65-2
      Alternate Names
      • EC:1.16.3.1
      • FTMT
      Background InformationFerritin, mitochondrial (UniProt: Q8N4E7; also known as EC:1.16.3.1, FTMT) is encoded by the FTMT gene (Gene ID: 94033) in human. Mitochondria are shown to be the sites where iron is transformed into heme and Fe-S-clusters. Mitochondrial ferritin (FtMt), a novel H-type ferritin, with ferroxidase activity. It is a homooligomeric protein with 24 subunits that is synthesized with a transit peptide (aa 1-49) that is subsequently cleaved off in the mitochondrial matrix to produce the mature protein (aa 50-242). The functional molecule is roughly spherical and contains a central cavity into which the polymeric mineral iron core is deposited. It stores iron in a soluble, non-toxic, readily available form. It is detected in testis and erythroleukemia and is also expressed in very low levels in the brain and other tissues. FtMt levels appear to correlate more with mitochondrial abundance than with iron metabolism. Higher levels of FtMt are reported in the cerebral cortex of Alzheimer s disease patients and in the substantia nigra of subjects with Parkinson s disease. Although FtMt exhibits about 79% homology to H-chain ferritin (FTH), Clone C65-2 specifically recognize only the FtMt and does not react with FTH. (Ref.: Yang, M., et al. (2017). Acta Histochem. Cytochem. 50(1); 49-55; Yang, H., et al. (2013). Neurosci. Res. 77(1-2); 1-7).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Mitochondrial ferritin/FTMT clone C65-2 , Cat. No. MABN2635, is a mouse monoclonal antibody that detects mitochondrial ferritin and is tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesTested Applications

      Immunohistochemistry Applications: A representative lot detected Mitochondrial ferritin/FTMT in Immunohistochemistry applications (Yang, M., et al. (2017). Acta Histochem Cytochem. 50(1):49-55).

      Western Blotting Analysis: A representative lot detected Mitochondrial ferritin/FTMT in Western Blotting applications (Yang, M., et al. (2017). Acta Histochem Cytochem. 50(1):49-55).

      Immunofluorescence Analysis: A representative lot detected Mitochondrial ferritin/FTMT in Immunofluorescence applications (Yang, M., et al. (2017). Acta Histochem Cytochem. 50(1):49-55).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 9 amino acids from the C-terminal region of human mitochondrial ferritin.
      EpitopeC-terminal
      CloneC65-2
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone C65-2 is a mouse monoclonal antibody that detects mitochondrial ferritin. It targets an epitope within nine amino acids from the C-terminal region.
      IsotypeIgMκ
      Species Reactivity
      • Human
      • Monkey
      Species Reactivity NoteHuman, Monkey.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FTMT
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~29 kDa observed; 27.54 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from HEK293 cells overexpressing Ferritin mitochondrial (FTMT).

      Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected Ferritin, mitochondrial (FTMT) in lysate from HEK293 cells overexpressing FTMT.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2635-25UG 04065267404059