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MAB4150 Anti-MRP2 Antibody, CT, clone M2 III-6

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MAB4150
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IHC, IH(P)MCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMAB4150
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MRP2 Antibody, CT, clone M2 III-6
      Alternate Names
      • cMOAT
      Product Information
      FormatCulture Supernatant
      PresentationLiquid containing 0.7% BSA and 0.1% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationDetect MRP2 using this Anti-MRP2 Antibody, C-terminus, clone M2 III-6 validated for use in WB, IC, IH, IH(P).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blotting: 1:20 - 1:50 with anti-mouse HRP

      Immunocytochemistry: 1:20 - 1:50 on acetone fixed cytospin preparations

      Immunohistochemistry: 1:20 on acetone fixed frozen tissue sections; 1:20 on formaldehyde-fixed paraffin-embedded tissues (pretreat with 0.01M citrate, pH 6.0, in dH2O at 100ºC for 5 minutes, repeated 3 times)

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenBacterial fusion protein of MRP2 containing the 202-aa C-terminal end
      EpitopeC-terminus
      CloneM2 III-6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody reacts with an internal epitope of MRP2, a 190-200 kDa transmembrane protein previously known as the canalicular multi-organic anion transporter (cMOAT). MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 over expression has been observed in a subset of cisplatin resistant cell lines. This antibody does not cross-react with the human MDR1, MRP1, MRP3 or MRP5 gene products.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.
      Gene Symbol
      • ABCC2
      • MRP2
      • ABC#
      • CMOAT1
      • CMOAT
      • ABC30
      • CMRP
      • DJS
      • KIAA1010
      • cMRP
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q92887 # Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
      SIZE: 1545 amino acids; 174191 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
      DISEASE: SwissProt: Q92887 # Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
      SIMILARITY: SwissProt: Q92887 ## Belongs to the ABC transporter family. Conjugate transporter (TC 3.A.1.208) subfamily. & Contains 2 ABC transmembrane type-1 domains. & Contains 2 ABC transporter domains.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C for up to 6 months. For long-term storage store at -20°C.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB4150 04053252359576