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MABN2304-25UG Anti-Legumain/Asparaginyl endopeptidase antibody Clone 6E3

Anti-LGMN/AEP, clone 6E3, Cat. No. MABN2304, is a mouse monoclonal antibody that detects Legumain and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

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MABN2304-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2304-25UG
      DescriptionAnti-Legumain/Asparaginyl endopeptidase antibody Clone 6E3
      Alternate Names
      • Asparaginyl endopeptidase
      • Protease cysteine 1
      Background InformationLegumain (UniProt: Q99538; also known as EC: 3.4.22.34, Asparaginyl endopeptidase, AEP, Protease cysteine 1, Delta Secretase) is encoded by the LGMN (also known as PRSC1) gene (Gene ID: 5641) in human. AEP is a lysosomal endopeptidase that displays strict specificity for the hydrolysis of asparaginyl bonds and can simultaneously cleave both amyloid precursor protein (APP) and tau. It cleaves aspartyl bonds slowly, especially under acidic conditions. AEP is synthesized with a signal peptide (aa 1-17) and a propeptide (aa 324-433) that are cleaved off in the mature form. It exists a homodimer before removal of the propeptide and after autocatalytic processing exists as a monomer. In its zymogen form, the uncleaved propeptide blocks access to the active site. AEP is phosphorylated on serine 226 by serine-arginine protein kinase 2 (SRPK2), which accelerates its autocatalytic cleavage and enhances its enzymatic activity. The active form translocates to cytoplasm. In Alzheimer s disease brains AEP is reported to be highly phosphorylated. Depletion of AEP is shown to reduce amyloid peptide production and senile plaque formation in 5XFAD mouse brain that leads to restoration of synaptic activity and cognitive function. Three isoforms of AEP have been described that are produced by alternative splicing. (Ref.: Wang Z-H., et al. (2017). Mol. Cell 67(5); 812-825; Zhang, Z et al (2015). Nat. Commun. 6; 9762).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-LGMN/AEP, clone 6E3, Cat. No. MABN2304, is a mouse monoclonal antibody that detects Legumain and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesTested Applications

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Legumain/AEP in mouse brain tissue lysate.

      ELISA Analysis: A representative lot detected Legumain/AEP in ELISA applications (Mattock, K.L., et al. (2010). Atherosclerosis.208(1):83-9; Kang, S.S., et al. (2018). EMBO J. 37(12):e98878).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated legumain/AEP in Immunoprecipitation applications (Zhang, Z., et al. (2015). Nat Commun. 6:8762).

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Legumain/AEP in human placenta tissue sections.

      Immunocytochemistry Analysis: A representative lot detected Legumain/AEP in Immunocytochemistry applications (Li, D.N., et al. (2003). J. Biol. Chem. 278(40):38980-90; Wang, Z-H., et al. (2019). Cell Rep. 28(3):655-669).

      Western Blotting Analysis: A representative lot detected Legumain/AEP in Western Blotting applications (Probst-Kepper, M., et al. (2009). J Cell Mol Med.;13(9B):3343-57; Zhang, Z., et al. (2015). Nat Commun.;6:8762; Wang, Z-H., et al. (2017). Mol Cell.;67(5):812-825; Zhang, Z., et al. (2017). Nat Struct Mol Biol.;24(8):632-642; Kang, S.S., et al. (2018). EMBO J.;37(12):e98878; Wang, Z-H., et al. (2018). Nat Commun.;9(1):1784; Wang, Z-H., et al. (2018). JCI Insight.;3(16):e99007; Wang, Z-H., et al. (2019). Cell Rep.;28(3):655-669).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged full-length recombinant human legumain.
      EpitopeUnknown
      Clone6E3
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 6E3 is a mouse monoclonal antibody that specifically detects Legumain (AEP).
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LGMN
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~55 kDa observed; 49.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SH-SY5Y cell lysate.

      Western Blotting Analysis: A 1:1000 dilution of this antibody detected Legumain/AEP in SH-SY5Y cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2304-25UG 04065267463889