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MABE71 Anti-Histone Antibody, Pan, clone F152.C25.WJJ

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MABE71
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, BWB, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE71
      ReplacesMAB052
      DescriptionAnti-Histone Antibody, Pan, clone F152.C25.WJJ
      Alternate Names
      • Histone H1.4
      • Histone H1b
      Background InformationThis antibody detects histone H1 and nuclesomal core proteins histones H2a, H2b, H3, H4. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      Histone H1 is a nuclear protein necessary for condensation of nucleosome chains into higher order structures.
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Histone Antibody, Pan, clone F152.C25.WJJ is a Mouse Monoclonal Antibody for detection of Histone also known as Histone H1.4, Histone H1b & has been validated in WB, ELISA.
      Key Applications
      • Western Blotting
      • ELISA
      Application NotesELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Mizoguchi, E., et al. (1997). Gastroenterology. 113:1828-1835.)
      Biological Information
      CloneF152C25W
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Bovine
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • HIST1H1E
      • H1F4
      Purification MethodProtein G
      UniProt Number
      Molecular Weight ~14-33 kDa observed
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H1 and core proteins on 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MABE71 04053252416682