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NE1015 Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb (SMI-22)

This Anti-Glial Fibrillary Acidic Protein Cocktail Mouse mAb is validated for use in ELISA, Frozen Sections, WB, ICC, Paraffin Sections for the detection of Glial Fibrillary Acidic Protein.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-GFAP Cocktail

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NE1015
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, Ch, Gp, H, M, Porcine, R, ShMMonoclonal Antibody

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NE1015-100UL
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      Description
      OverviewRecognizes ~50 kDa glial fibrillary acidic protein (GFAP) in human and bovine cytoskeletal preparations.
      Catalogue NumberNE1015
      Brand Family Calbiochem®
      SynonymsAnti-GFAP Cocktail
      Application Data
      Detection of rat glial fibrillary acidic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-GFAP Cocktail Mouse mAb (SMI-22) (Cat. No. NE1015) (1:1000). Detection: fluorescence (red) with Hoechst 33342 counterstain.

      Primary mixed glial cultures were stained with anti-mouse GFAP (EMD Millipore, Cat. No. MAB360) and a DyLight™488 conjugated goat anti-mouse secondary Antibody. The nucleus was counterstained with DAPI. The image was captured using Olympus BX51 with an exposure time of 982 millisec for green channel and 126 millisec for DAPI channel. Magnification is 10X without any correction on Gain/Offset/Bad pixel. Courtesy of Jose Shinsmon, Immunology, Dept of Pathology, Faculty of Medicine and Health Sciences, UPM,Serdang 43400.
      References
      ReferencesVick, W.W., et al. 1987. Acta. Cytol. 31, 816.
      McLendon R.E., et al. 1986. J. Neuropathol. Exp. Neurol. 45, 692.
      Pegram, C.N., et al. 1985. Neurochem. Pathol. 3, 119.
      Product Information
      FormLiquid
      FormulationUndiluted ascites.
      Positive controlAstrocytes or cytoskeletal preparations
      Preservative≤ 0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (1:1000)
      Frozen Sections (1:1000, see comments)
      Immunoblotting (1:1000)
      Immunocytochemistry (1:1000, see comments)
      Paraffin Sections (1:1000, trypsin or heat pre-treatment required)
      Application CommentsThis cocktail is derived from the Bigner-Eng clones MAb1B4, MAb2E1, and MAb4A11 and provides a means for more comprehensive detection of astrocytomas than each clone alone. Each component is specific for GFAP and stains astrocytes and astrocytic processes as well as Bergman glia. Recognizes both anaplastic and reactive astrocytes by immunocytochemical staining. Does not recognize metastatic tumors and brain tumors of non-astrocytic origin, including medulloblastomas, meningiomas, choroid plexus papillomas, and schwannomas. For staining paraffin sections it is recommended that de-paraffinized sections be treated with 0.1% trypsin in 50 mM Tris-HCl, pH 7.6 for 20-30 min at 37°C or boiled in Tris-buffered saline, pH 9.0 for 15 min to expose the epitope. For immunocytochemistry or staining frozen sections, post-fixation in cold methanol or methanol/hydrogen peroxide for 10 min is required for access to the astrocytes in the sample. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified bovine GFAP protein
      ImmunogenBovine
      CloneSMI-22
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Bovine
      • Canine
      • Chicken
      • Guinea Pig
      • Human
      • Mouse
      • Porcine
      • Rat
      • Sheep
      Antibody TypeMonoclonal Antibody
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsUpon initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      NE1015-100UL 04055977209853