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DR1066 Anti-GTF2I Rabbit pAb

DR1066
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~130 kDa GTF2I protein.
      Catalogue NumberDR1066
      Brand Family Calbiochem®
      SynonymsAnti-General Transcription Factor IIi
      Application Data
      Detection of human GTF2I by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from cells transfected with full-length human GTF2I. Antibody for IP: Anti-GTF2I Rabbit pAb (Cat. No. DR1066) (ratio of Ab to protein A beads 1:50-1:00). Antibody for IB: Anti-GTF2I Mouse pAb. Detection: chemiluminescence.
      References
      Product Information
      FormulationUndiluted serum.
      PreservativeNone
      Applications
      Key Applications Immunoprecipitation
      Application NotesImmunoprecipitation (see comments)
      Application CommentsFor immunoprecipitation, the recommended ratio between antibody and protein A beads is 1:50 to 1:100; the amount of antibody is sample dependent. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, human GTF2I (aa 1-976)
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1066 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-General Transcription Factor IIi
      ApplicationImmunoprecipitation (see comments)
      Application Data
      Detection of human GTF2I by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from cells transfected with full-length human GTF2I. Antibody for IP: Anti-GTF2I Rabbit pAb (Cat. No. DR1066) (ratio of Ab to protein A beads 1:50-1:00). Antibody for IB: Anti-GTF2I Mouse pAb. Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~130 kDa GTF2I protein.
      BackgroundGTF2l is required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
      HostRabbit
      Immunogen speciesHuman
      ImmunogenFull-length, human GTF2I (aa 1-976)
      IsotypeIgG
      Specieshuman
      FormulationUndiluted serum.
      PreservativeNone
      CommentsFor immunoprecipitation, the recommended ratio between antibody and protein A beads is 1:50 to 1:100; the amount of antibody is sample dependent. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review