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ABN1464 Anti-GRP-78

Anti-GRP-78, Cat. No. ABN1464, is a highly specific rabbit polyclonal antibody that targets 78 kDa glucose-regulated protein and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-GRP-78 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: HSPA5, GRP78 UniProt Number: P11021
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ABN1464
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), WB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1464
      DescriptionAnti-GRP-78
      Alternate Names
      • 78 kDa glucose-regulated protein
      • GRP-78
      • Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
      • Heat shock 70 kDa protein 5
      • Immunoglobulin heavy chain-binding protein
      • BiP
      Background Information78 kDa glucose-regulated protein (UniProt: P11021; also known as GRP-78, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70 kDa protein 5, Immunoglobulin heavy chain-binding protein, BiP) is encoded by the HSPA5 (also known as GRP78) gene (Gene ID: 3309) in human. GRP78 a member of the HSP family of molecular chaperones that is required for stress-induced autophagy and for endoplasmic reticulum (ER) integrity. GRP78 is involved in multiple processes, including translocation of the newly formed proteins across the ER membrane, folding and assembly of proteins, and targeting misfolded proteins for ER-associated degradation. It plays a critical role in regulating unfolded protein response (UPR). Following ER stress, GRP78 is released from ER transmembrane signal transducers, such as PKR-like ER kinase (PERK), which leads to the activation of UPR signaling pathways. Once GRP78 is dissociated, PERK is allowed to dimerize and is autophosphorylated and activated. Activated PERK phosphorylates elF2alpha that results in the reduction of protein synthesis. (Ref.:Wang, M., et al. (2009). Antioxid Redox Signal. 11(9): 2307 2316).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GRP-78, Cat. No. ABN1464, is a highly specific rabbit polyclonal antibody that targets 78 kDa glucose-regulated protein and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 0.2 µg/mL from a representative lot detected GRP-78 in 10 µg of A431 cell lysates.

      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GRP-78 in human cerebellum, human pancreas, human small intestine, and human tonsil tissues.

      Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GRP-78 in NIH/3T3 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human GRP-78.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects GRP-78 protein in multiple mammalian species. It targets an epitope within 17 amino acids from the C-terminal region.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Goat, Ground Squirrel, Hamster, Mouse, and Primates based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HSPA5
      • GRP78
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~78 kDa observed; 72.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysates.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected GRP-78 in 10 µg of A431 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1464 04054839199332