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AP1036 Anti-F₁F₀-α Mouse mAb (7H10BD4F9)

Anti-F₁F₀-α Mouse mAb (7H10BD4F9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
AP1036
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the F1F0-α subunit of Complex V in bovine heart mitochondria.
      Catalogue NumberAP1036
      Brand Family Calbiochem®
      Application Data
      Detection of bovine F1F0-α by immunoblotting. Sample: F1F0-α immunoprecipitated from heart mitochondria. Primary antibody: Anti-F1F0-α Mouse mAb (7H10BD4F9) (Cat. No. AP1036) (1 μg/ml). Detection: chemiluminescence.

      Detection of human F1F0-α by immunocytochemistry. Sample: 143B osteosarcoma cells. Primary antibody: Anti-F1F0-α Mouse mAb (7H10BD4F9) (Cat. No. AP1036) (5 μg/ml). Detection: fluorescence.
      References
      ReferencesMoser, T.L., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 6656.
      Osanai, T., et al. 2001. J. Clin. Invest. 108, 1023.
      Moser, T.L., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 2811.
      Osanai, T., et al. 1998. J. Biol. Chem. 273, 31778.
      Product Information
      EC number3.6.1.14
      FormLiquid
      FormulationIn HEPES-buffered saline (HBS).
      Positive controlHeart mitochondria
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Cell Inhibition Assays
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Cell Inhibition Assays (see comments)
      Application CommentsThis antibody has been shown to act as an angiostatin mimetic and to inhibit the proliferation of endothelial cells. Not all cells express F1F0 on the surface so not all endothelial cells will stain positively for surface expression. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenbovine Complex V
      ImmunogenBovine
      Clone7H10BD4F9
      HostMouse
      IsotypeIgG2b
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1036 0

      Documentation

      Anti-F₁F₀-α Mouse mAb (7H10BD4F9) Certificates of Analysis

      TitleLot Number
      AP1036

      References

      Reference overview
      Moser, T.L., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 6656.
      Osanai, T., et al. 2001. J. Clin. Invest. 108, 1023.
      Moser, T.L., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 2811.
      Osanai, T., et al. 1998. J. Biol. Chem. 273, 31778.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-July-2009 RFH
      ApplicationImmunoblotting (1 µg/ml)
      Immunocytochemistry (5 µg/ml)
      Cell Inhibition Assays (see comments)
      Application Data
      Detection of bovine F1F0-α by immunoblotting. Sample: F1F0-α immunoprecipitated from heart mitochondria. Primary antibody: Anti-F1F0-α Mouse mAb (7H10BD4F9) (Cat. No. AP1036) (1 μg/ml). Detection: chemiluminescence.

      Detection of human F1F0-α by immunocytochemistry. Sample: 143B osteosarcoma cells. Primary antibody: Anti-F1F0-α Mouse mAb (7H10BD4F9) (Cat. No. AP1036) (5 μg/ml). Detection: fluorescence.
      DescriptionMouse monoclonal antibody purified by ammonium sulfate precipitation. Recognizes F1F0α subunit of Complex V of the mitochondrial respiratory chain/oxidative phosphorylation system.
      BackgroundComplex V of the respiratory chain/oxidative phosphorylation system (also called F1F0 ATPase) couples the passage of protons across the intermembrane space to the synthesis of ATP from ADP and Pi. The enzyme was originally thought to be localized exclusively to mitochondria. However, it also reported to be expressed on the plasma membrane of several cell types including hepatocytes where it is the HDL receptor, on endothelial cells where it may act as the angiostatin receptor, and on the surface of cancer cells. The enzyme in mammals is composed of 17 subunits, 5 of which make up the easily detached F1. The remainder are components of 2 stalk domains and the proton pumping F0 part of the machinery. Two of the subunits of the F0 part are encoded on mtDNA while the other subunits are nuclear encoded. Mutations in the mtDNA-encoded subunits of Complex V cause OXPHOS disease.
      HostMouse
      Immunogen speciesBovine
      Immunogenbovine Complex V
      Clone7H10BD4F9
      IsotypeIgG2b
      Speciesbovine, human, mouse, rat
      Positive controlHeart mitochondria
      FormLiquid
      FormulationIn HEPES-buffered saline (HBS).
      Concentration Label Please refer to vial label for lot-specific concentration
      EC number3.6.1.14
      Preservative≤0.1% sodium azide
      CommentsThis antibody has been shown to act as an angiostatin mimetic and to inhibit the proliferation of endothelial cells. Not all cells express F1F0 on the surface so not all endothelial cells will stain positively for surface expression. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMoser, T.L., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 6656.
      Osanai, T., et al. 2001. J. Clin. Invest. 108, 1023.
      Moser, T.L., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 2811.
      Osanai, T., et al. 1998. J. Biol. Chem. 273, 31778.