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ABC1378-25UG Anti-EZHIP

Anti-EZHIP, Cat. No. ABC1378, is a rabbit polyclonal antibody that detects EZH inhibitory protein (EZHIP, CXorf67) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

ABC1378-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABC1378-25UG
      DescriptionAnti-EZHIP
      Alternate Names
      • EZH inhibitory protein
      • Enhancer of Zeste Homologs Inhibitory Protein
      Background InformationEZH inhibitory protein (UniProt: Q86X51; also known as EZHIP) is encoded by the EZHIP (also known as CXorf67) gene (Gene ID: 340602) in human. EZHIP is expressed predominantly in the gonads. In testis, it is detected in male germ cells inside the seminiferous tubules, especially in spermatogonia and round spermatids. In the ovary, it is expressed in primordial follicles and oocytes but not the external follicle cells. It is shown to inhibit PRC2/EED-EZH1 and PRC2/EED-EZH2 complex function by inhibiting EZH1/EZH2 methyltransferase activity, thereby causing down-regulation of histone H3 trimethylation on lysine 27 (H3K27me3). It inhibits H3K27me3 deposition during spermatogenesis and oogenesis. Its inhibitory effect on methyltransferase activity is attributed to its ability to limit the stimulatory effect of cofactors such as AEBP2 and JARID2. Amino acid sequence 403-423 is reported to be essential and sufficient for inhibition of PRC2/EED-EZH1 and PRC2/EED-EZH2 complex activity and the sequence 401-409 is sufficient for its interaction with EZH2. (Ref.: Ragazzini, R., et al. (2019). Nat. Commun. 10(1); 3858).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-EZHIP, Cat. No. ABC1378, is a rabbit polyclonal antibody that detects EZH inhibitory protein (EZHIP, CXorf67) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected EZHIP in human testis tissue sections.

      Western Blotting Analysis: A representative lot detected EZHIP in Western Blotting applications (Jain, SU et al. (2020), S1097-2765(20); 30680-8.

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected EZHIP in HEK293T FLAG-EZHIP (Courtesy of Dr. Peter Lewis Lab @ University of Wisconsin).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal half of human EZH Inhibitory protein.
      EpitopeC-terminal half
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects EZH inhibitory protein (EZHIP).
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Monkey, Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EZHIP
      • CXorf67
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~70 kDa observed; 51.89 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293T cells transfected with FLAG-EZHIP.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected EZH inhibitory protein (CXorf67) in lysate from HEK293T cells transfected with FLAG-EZHIP.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC1378-25UG 04065265651462