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AP1046 Anti-CtIP Rabbit pAb

Anti-CtIP Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
AP1046
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~125 kDa CtIP protein in MCF7 cells.
      Catalogue NumberAP1046
      Brand Family Calbiochem®
      Application Data
      Lane 1: Detection of CtIP by immunoblotting. Sample: Whole cell lysate (50 µg) from MCF7 cells. Primary antibody: Anti-CtIP Rabbit pAb (Cat. No. AP1046) (1 µg/ml). Detection: chemiluminescence. Lane 2/3: Detection of CtIP by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from MCF7 cells. Antibodies for IP: Anti-CtIP Rabbit pAb (Cat. No. AP1046) (3 µg/mg protein) (lane 2) and Rabbit IgG (3 µg/mg protein) (lane 3). Immunoblotting conditions: same as above.
      References
      ReferencesChen, P.L., et al. 2005. Mol. Cell Biol. 25, 3535.
      Yu, X. and Chen, J. 2004. Mol. Cell Biol. 24, 9478.
      Germani, A., et al. 2003. Oncogene 22, 8845.
      Li, S., et al. 2000. Nature 406, 210.
      Wong, A.K., et al. 1998. Oncogene 17, 2279.
      Product Information
      FormLiquid
      FormulationIn Tris citrate/phosphate buffer, pH 7-8.
      Positive controlMCF7 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml)
      Immunoprecipitation (3 µg/mg protein)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human CtIP
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1046 0

      Documentation

      Anti-CtIP Rabbit pAb Certificates of Analysis

      TitleLot Number
      AP1046

      References

      Reference overview
      Chen, P.L., et al. 2005. Mol. Cell Biol. 25, 3535.
      Yu, X. and Chen, J. 2004. Mol. Cell Biol. 24, 9478.
      Germani, A., et al. 2003. Oncogene 22, 8845.
      Li, S., et al. 2000. Nature 406, 210.
      Wong, A.K., et al. 1998. Oncogene 17, 2279.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      ApplicationImmunoblotting (1 µg/ml)
      Immunoprecipitation (3 µg/mg protein)
      Application Data
      Lane 1: Detection of CtIP by immunoblotting. Sample: Whole cell lysate (50 µg) from MCF7 cells. Primary antibody: Anti-CtIP Rabbit pAb (Cat. No. AP1046) (1 µg/ml). Detection: chemiluminescence. Lane 2/3: Detection of CtIP by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from MCF7 cells. Antibodies for IP: Anti-CtIP Rabbit pAb (Cat. No. AP1046) (3 µg/mg protein) (lane 2) and Rabbit IgG (3 µg/mg protein) (lane 3). Immunoblotting conditions: same as above.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~125 kDa CtIP protein in MCF7 cells.
      BackgroundCtBP interacting protein (CtIP; Accession No. NP_002885), originally identified as a binding partner of the transcriptional suppressor CtBP, was subsequently shown by two-hybrid screening to interact with BRCA1 BRCT domains. CtIP is a phosphorylation-dependent binding partner of the BRCA1 BRCT domains. The cell cycle-regulated BRCA1/CtIP complex is required for the G2/M transition and Chk1 activation following DNA damage. CtIP interacts with a group of tumor suppressor proteins including Rb (retinoblastoma protein), BRCA1, Ikaros, and CtBP, which regulate cell cycle progression through transcriptional repression as well as chromatin remodeling. CtIP associates with SIAH-1 both in vitro and in vivo leading to CtIP degradation by the ubiquitin-proteasome pathway. CtIP(-/-) embryos die at embryonic day 4 with blastocysts arrested in G1, in an Rb dependent manner. CtIP(+/-) heterozygotes are characterized by the development of multiple types of tumors, predominantly, large lymphomas. CtIP becomes hyperphosphorylated and dissociated from BRCA1 upon exposure to ionizing radiation. This phosphorylation event requires the protein kinase, ATM, that is mutated in the disease ataxia telangiectasia. ATM phosphorylates CtIP at Ser664 and Ser745. ATM modulates BRCA1-mediated regulation of the DNA damage-response GADD45 gene through CtIP, thus providing a potential link between ATM deficiency and breast cancer.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human CtIP
      IsotypeIgG
      Specieshuman
      Positive controlMCF7 cells
      FormLiquid
      FormulationIn Tris citrate/phosphate buffer, pH 7-8.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesChen, P.L., et al. 2005. Mol. Cell Biol. 25, 3535.
      Yu, X. and Chen, J. 2004. Mol. Cell Biol. 24, 9478.
      Germani, A., et al. 2003. Oncogene 22, 8845.
      Li, S., et al. 2000. Nature 406, 210.
      Wong, A.K., et al. 1998. Oncogene 17, 2279.