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MAB3501 Anti-Caspase 2 Antibody, clone 10C6

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MAB3501
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, M, Mk, RELISA, FC, IP, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3501
      Replaces04-571
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Caspase 2 Antibody, clone 10C6
      Alternate Names
      • ICH-1
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody in buffer containing PBS, 0.1% Kathon.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Caspase 2 Antibody, clone 10C6 detects level of Caspase 2 & has been published & validated for use in ELISA, FC, IP & WB.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot: Recommended usage at 5 μg/ml, which has been found to detect endogenous Caspase-2 in 5 x 10E5 HeLa and MCF-7 cells.

      Immunoprecipitation: Recommended concentration 1-5 μg/ml FACS: Recommended concentration 1 μg/ml. It is necessary to permeabilize the cell membrane prior to analysis.

      EIA: Recommended concentration 0.5-1 μg/ml.

      Immunohistochemistry: Use at 10-20 μg/ml. It is necessary to fix the cells in 4% Paraformaldehyde/0.18% Triton-X100 for 10 minutes prior to staining.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRaised against the P19 subunit of recombinant human Caspase-2 protein.
      Clone10C6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      SpecificityMAB3501 antibody recognizes a reactive epitope that lies in the P19 subunit of human Caspase-2 (ICH-1, Nedd-2).
      IsotypeIgG2a
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Monkey
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.
      Gene Symbol
      • CASP2
      • NEDD2
      • ICH1
      • NEDD-2
      • ICH-1L/1S
      • CASP-2
      • ICH-1L
      • EC 3.4.22.55 [Contains: Caspase-2 subunit p18
      • Caspase-2 subunit p13
      • Caspase-2 subunit p12].
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42575 # Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
      SIZE: 452 amino acids; 50685 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD.
      TISSUE SPECIFICITY: Expressed in larger amounts in the embryonic lung, liver and kidney than in the heart and brain. In the adults higher level expression is seen in the placenta, lung, kidney, pancreas than in the heart, brain, liver and skeletal muscle.
      PTM: The mature protease can process its own propeptide, but not that of other caspases.
      SIMILARITY: SwissProt: P42575 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3501 04053252660849