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ABS1507 Anti-Aurora B

Anti-Aurora B, Cat. No. ABS1507, is a rabbit polyclonal antibody that detects Aurora Kinase B and has been tested for use in Immunocytochemistry and Western Blotting.

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ABS1507
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS1507
      DescriptionAnti-Aurora B
      Alternate Names
      • Aurora kinase B
      • EC:2.7.11.1
      • Aurora 1
      • Aurora- and IPL1-like midbody-associated protein 1
      • AIM-1
      • Aurora/IPL1-related kinase 2
      • ARK-2
      • Aurora-related kinase 2
      • STK-1
      • Serine/threonine-protein kinase 12
      • Serine/threonine-protein kinase 5
      • Serine/threonine-protein kinase aurora-B
      Background InformationAurora kinase B (UniProt: Q96GD4; also known as EC:2.7.11.1, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, ARK-2, Aurora-related kinase 2, STK-1, Serine/threonine-protein kinase 12, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase aurora-B) is encoded by the AURKB (also known as AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5) gene (Gene ID: 9212) in human. Aurora Kinase B is a serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. It is a key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Aurora kinase B phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP and phosphorylation of INCENP leads to increased Aurora kinase B activity. Five isoforms of Aurora Kinase are reported that are produced by alternative splicing. Its expression is shown to be cell-cycle regulated, with low expression in G1/S phase and an increase during G2 and M phase. Following M phase its expression declines. Cancer cells display high expression of Aurora kinase B during M phase. Disruption in regulation of Aurora kinase B causes perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis. High level expression is observed in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Substitution of Lysine 106 with arginine leads to loss of its kinase activity and severely impairs mitotic progression.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Aurora B, Cat. No. ABS1507, is a rabbit polyclonal antibody that detects Aurora Kinase B and has been tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Aurora B in HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated Linear Peptide corresponding to the 11 amino acids from the C-terminal region of Human Aurora kinase B.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibdy detects Aurora kinase B in human cells.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AURKB
      • AIK2
      • AIM1
      • AIRK2
      • ARK2
      • STK1
      • STK12
      • STK5
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~39 kDa observed; 39.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Aurora B in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1507 04054839412363

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