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MABN2273 Anti-Amyloid Beta A4 protein Antibody, clone 2D8

Anti-Amyloid Beta A4 protein, clone 2D8 Antibody, Cat. No. MABN2273, is a highly specific rat monoclonal antibody that targets Amyloid beta A4 protein and has been tested in Immunoprecipitation and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: APP, A4, AD1 UniProt Number: P05067
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MABN2273
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, IPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2273
      DescriptionAnti-Amyloid Beta A4 protein Antibody, clone 2D8
      Alternate Names
      • ABPP
      • APPI
      • APP
      • Alzheimer disease amyloid protein
      • Amyloid precursor protein
      • Beta-amyloid precursor protein
      • Cerebral vascular amyloid peptide
      • CVAP
      • PreA4
      • Protease nexin-II
      • PN-II
      Background InformationAmyloid beta A4 protein (UniProt: P05067; also known as ABPP, APPI. APP, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Beta-amyloid precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. Deposition of Amyloid beta (Ab) is an early event in the pathogenesis of Alzheimer s disease (AD). Ab peptides originate from the proteolytic cleavage of the amyloid precursor protein (APP). APPs are cell surface protein that are internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha-secretase cleavage, soluble APP is released into the extracellular space and the C-terminal is internalized to endosomes and lysosomes. The beta-secretase cleaves APP between residues Met671 and Asp672 and yields sAPP beta and C99. Following the beta-secretase cleavage, a second cleavage occurs at the C-terminus of Ab peptide that releases Ab from C99. This cleavage occurs in the vicinity of residue 712 of the C-terminus. The gamma-secretase can cleave the C-terminal region at either Val711 or Ile713 to produce the shorter Ab peptide (Ab1-40) or the longer Ab peptide (Ab1-42). Ab1-42 occurs more frequently and forms fibrillar aggregates far more readily than the Ab1-40 peptide. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu2+ and Fe3+ to Cu+ and Fe2+, respectively. Abeta42 is a more effective reductant than beta-amyloid 40. From a physiological point of view, it functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. It is also involved in cell mobility and transcription regulation through protein-protein interactions and can promote transcription activation through binding to APBB1-KAT5.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Amyloid Beta A4 protein, clone 2D8 Antibody, Cat. No. MABN2273, is a highly specific rat monoclonal antibody that targets Amyloid beta A4 protein and has been tested in Immunoprecipitation and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: 0.5 ug/mL from a representative lot detected Amyloid Beta A4 protein in 10 µg of N2aA transfected, N2a murine neuroblastoma, and N2aAB treated cell lysate.

      Immunoprecipitation Analysis: A representative lot detected Amyloid Beta A4 protein in Immunoprecipitation applications (Willem, M., et. al. (2015). Nature. 526(7573):443-7).

      Western Blotting Analysis: A representative lot detected Amyloid Beta A4 protein in Western Blotting applications (Willem, M., et. al. (2015). Nature. 526(7573):443-7).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human Amyloid beta A4 protein.
      Clone2D8
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 2D8 deects Amyloid beta A4 protein in human and murine neuroblastoma cells. It targets an epitope within 16 amino acids fromt the C-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APP
      • A4
      • AD1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~110 kDa observed; 86.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human Neuroblastoma (Ntera-2) lysate.

      Western Blotting Analysis: 0.5 ug/mL of this antibody detected Amyloid Beta A4 protein in 10 µg of human Neuroblastoma (Ntera-2) cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2273 04054839198984

      Documentation

      Anti-Amyloid Beta A4 protein Antibody, clone 2D8 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Amyloid Beta A4 protein Antibody, clone 2D8 Certificates of Analysis

      TitleLot Number
      Anti-Amyloid Beta A4 protein, clone 2D8 - 3855725 3855725
      Anti-Amyloid Beta A4 protein, clone 2D8 - 4003924 4003924
      Anti-Amyloid Beta A4 protein, clone 2D8 - 4157294 4157294
      Anti-Amyloid Beta A4 protein, clone 2D8 - 4186000 4186000
      Anti-Amyloid Beta A4 protein, clone 2D8 - 4187673 4187673
      Anti-Amyloid Beta A4 protein, clone 2D8, Monoclonal Antibody Q2841554