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MABN59 Anti-ACE2 Antibody, clone 4G5.1

Anti-ACE2 Antibody, clone 4G5.1 detects level of ACE2 & has been validated for use in Western Blotting.

Anti-ACE2 Antibody, clone 4G5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: ACE2 UniProt Number: Q9BYF1
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MABN59
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN59
      ReplacesMAB5676
      DescriptionAnti-ACE2 Antibody, clone 4G5.1
      Alternate Names
      • Angiotensin-converting enzyme 2
      • ACE-related carboxypeptidase
      • Angiotensin-converting enzyme homolog
      • ACEH
      • Metalloprotease MPROT15
      Background InformationACE2 (Angiotensin-converting enzyme 2) is a transmembrane protein belonging to the peptidase M2 family. It is expressed in vascular endothelial cells of the heart, kidney, testis, and gastrointestinal system. ACE2 produces Angiotensin (1-9) by hydrolysis of Angiotensin 1. Angiotensin (1-9) is a precursor for Angiotensin (1-7). ACE2 may also directly produce Angiotensin (1-7) by conversion of Angiotensin II, which is involved in vasoconstriction. Angiotensin (1-7) plays an important role in the RAS signaling pathway and is involved in vasodilation. Hence, ACE2 antagonizes the effects of its homolog, ACE. ACE2 is insensitive to commonly used inhibitors such as linosipril and captopril. ACE2 is upregulated in heart failure, possibly in response to overactivity of Angiotensin II; ACE2 is studied for its potential therapeutic value for hypertension and heart failure.
      References
      Product Information
      FormatPurified
      Control
      • HEK293 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ACE2 Antibody, clone 4G5.1 detects level of ACE2 & has been validated for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesELISA Analysis: A representative lot detected ACE2 in a direct ELISA.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human ACE2.
      Clone4G5.1
      HostMouse
      SpecificityThis antibody recognizes ACE2.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
      Gene Symbol
      • ACE2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.

      CATALYTIC ACTIVITY: Angiotensin II + H2O = angiotensin-1-7 + L-phenylalanine.

      COFACTOR: Binds 1 zinc ion per subunit. Binds 1 chloride ion per subunit.

      ENZYME REGULATION: Activated by chloride and fluoride, but not bromide. Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat.

      SUBUNIT STRUCTURE: Interacts with ITGB1. Interacts with SARS-CoV and HCoV-NL63 spike glycoprotein.

      SUBCELLULAR LOCATION: Processed angiotensin-converting enzyme 2: Secreted. Cell membrane; Single-pass type I membrane protein.

      TISSUE SPECIFICITY: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.

      INDUCTION: Up-regulated in failing heart.

      POST-TRANSLATIONAL MODIFICATION: N-glycosylation on Asn-90 may limit SARS infectivity.

      SEQUENCE SIMILARITIES: Belongs to the peptidase M2 family.
      Molecular Weight~62 kDa observed. Uniport describes 2 isoforms at ~92 kDa (Isoform 1) and ~64 kDa (Isoform 2) due to alternative splicing.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate.

      Western Blot Analysis: A 1:2,000 dilution of this antibody detected ACE2 in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN59 04053252643415

      Documentation

      Anti-ACE2 Antibody, clone 4G5.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-ACE2 Antibody, clone 4G5.1 Certificates of Analysis

      TitleLot Number
      Anti-ACE2, clone 4G5.1 2487732
      Anti-ACE2, clone 4G5.1 - 2031360 2031360
      Anti-ACE2, clone 4G5.1 - 3257585 3257585
      Anti-ACE2, clone 4G5.1 - 3439690 3439690
      Anti-ACE2, clone 4G5.1 - 3451422 3451422
      Anti-ACE2, clone 4G5.1 - 3465732 3465732
      Anti-ACE2, clone 4G5.1 - 3476046 3476046
      Anti-ACE2, clone 4G5.1 - 3499346 3499346
      Anti-ACE2, clone 4G5.1 - 3508402 3508402
      Anti-ACE2, clone 4G5.1 - 3537157 3537157
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