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NA02 Sigma-AldrichAnti-2,2,7-Trimethylguanosine Mouse mAb (K121)

This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) is validated for use in Affinity Purification, ICC, IP, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

CoA
References
Brochures
Data Sheet

Recommended Products

Overview

Replacement Information

Key Spec Table

Species Reactivity	Host	Antibody Type
H, M, R	M	Monoclonal Antibody

Pricing & Availability

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Description
Overview	Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
Catalogue Number	NA02
Brand Family	Calbiochem®
Application Data	Detection of the 5' cap of snRNA by immunocytochemistry. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) (Cat. No. NA02) (0.8 μg/ml). Detection: fluorescence.

References
References	Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760. Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415. Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1. Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725. Kramer, A., et al. 1984. Cell 38, 299. Lerner, M.R., et al. 1980. Nature 283, 220.

Product Information
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative control	Transfer RNA (tRNA)
Positive control	Heterogenous nuclear RNA (hnRNA)
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Affinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599. Paraffin Sections Volm, M., et al. 2000. Clin. Cancer Res. 6, 3236.
Key Applications	Immunocytochemistry Immunoprecipitation Paraffin Sections
Application Notes	Affinity Purification (use Cat. No. NA02A, see application references) Immunocytochemistry (0.5-5 µg/ml) Immunoprecipitation (1-2 µg, see application references) Paraffin Sections (see application references)
Application Comments	Also recognizes 7-methylguanosine-capped mRNA. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography (use Cat. No. NA02A). All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	2,2,7-trimethylguanosine
Clone	K121
Host	Mouse
Isotype	IgG₁
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
NA02-100UG	07790788053840

Documentation

Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Certificates of Analysis

Title	Lot Number
NA02	Enter Lot Number

References

Reference overview
Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760. Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415. Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1. Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725. Kramer, A., et al. 1984. Cell 38, 299. Lerner, M.R., et al. 1980. Nature 283, 220.

Brochure

Title
Antibody Sourcebookﾙ Sixth Edition

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	04-September-2007 RFH
Application	Affinity Purification (use Cat. No. NA02A, see application references) Immunocytochemistry (0.5-5 µg/ml) Immunoprecipitation (1-2 µg, see application references) Paraffin Sections (see application references)
Application Data	Detection of the 5' cap of snRNA by immunocytochemistry. Sample: HeLa cells fixed with methanol. Primary antibody: Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) (Cat. No. NA02) (0.8 μg/ml). Detection: fluorescence.
Description	Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs).
Background	The U small nuclear ribonucleoprotein particles (snRNPs) are present in all eukaryotes studied thus far and are involved in the splicing of RNA molecules. snRNPs are composed of several proteins and one or two RNA molecules. The RNA molecules (snRNAs) contain a unique 5' terminal cap structure containing the nucleoside 2,2,7-trimethylguanosine. The 70 kDa protein component of U1 snRNP is cleaved during apoptosis, perhaps by a caspase. Monoclonal antibodies to the 5' cap structure react only to snRNAs.
Host	Mouse
Immunogen	2,2,7-trimethylguanosine
Clone	K121
Isotype	IgG₁
Species	human, mouse, rat
Positive control	Heterogenous nuclear RNA (hnRNA)
Negative control	Transfer RNA (tRNA)
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Also recognizes 7-methylguanosine-capped mRNA. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography (use Cat. No. NA02A). All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Casciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760. Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415. Reddy, R., and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1. Krainer, A.R., and Maniatis, T. 1985. Cell 42, 725. Kramer, A., et al. 1984. Cell 38, 299. Lerner, M.R., et al. 1980. Nature 283, 220.
Application references	Affinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599. Paraffin Sections Volm, M., et al. 2000. Clin. Cancer Res. 6, 3236.

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