71339 Sigma-AldrichpIEx™-7 Ek/LIC Vector Kit - Novagen
Novagen's pIEx-7 Ek/LIC Vector is designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus.
More>> Novagen's pIEx-7 Ek/LIC Vector is designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. Less<<pIEx™-7 Ek/LIC Vector Kit - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Synonyms: InsectDirect™ pIEx™-7 Ek/LIC Vector Kit
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Overview
| Replacement Information |
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Products
| Catalogue Number | Packaging | Qty/Pack | |
|---|---|---|---|
| 71339-3 | Plastová ampulka | 20 rxn |
| Description | |
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| Overview | The pIEx™-7 Ek/LIC Vector is a ligation-independent cloning-prepared derivative of the pIEx-6 vector designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells and a minimal N-terminal His•Tag® coding sequence. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. The Ek/LIC cloning site is designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional S•Tag™ coding sequence is present at the 3′ end of the MCS for generating a carboxy-terminal tag compatible with purification, detection and quantification (1). |
| This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. | |
| Catalogue Number | 71339 |
| Brand Family | Novagen® |
| Synonyms | InsectDirect™ pIEx™-7 Ek/LIC Vector Kit |
| References | |
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| References | Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356. |
| Product Information | ||
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| • | 1 µg | Ek/LIC Vector |
| • | 25 units | T4 DNA Polymerase, LIC-qualified |
| • | 50 µl | 25 mM EDTA |
| • | 40 µl | 25 mM dATP |
| • | 8 µl | Ek/LIC β-gal Control Insert |
| • | 100 µl | 100 mM DTT |
| • | 50 µl | 10X T4 DNA Polymerase Buffer |
| • | 1.5 ml | Nuclease-free Water |
| • | 5 × 2 ml | SOC Medium |
| • | 10 µl | Test Plasmid |
| • | 22 × 50 µl | NovaBlue GigaSingles™ Competent Cells |
| Quality Level | MQ100 | |
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| Safety Information according to GHS |
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| Safety Information |
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| Product Usage Statements |
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| Storage and Shipping Information | |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | ≤ -70°C |
| Do not freeze | Ok to freeze |
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| Specifications |
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| Global Trade Item Number | |
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| Catalogue Number | GTIN |
| 71339-3 | 04055977253269 |
Documentation
pIEx™-7 Ek/LIC Vector Kit - Novagen Certificates of Analysis
| Title | Lot Number |
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| 71339 |
References
| Reference overview |
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| Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356. |
Brochure
| Title |
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| Insect Cell Expression |
| Insect Directﾙ Protein Expression & Purification System |
| Radiance! Multisystem Protein Expression |
Citations
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User Protocols
| Title |
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| TB163 Ek/LIC Cloning Kits |
Vector Map
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| TB399VM pIEx-7 Ek/LIC Vector Map |
Vector Sequence
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| pIEx-7 Ek/LIC Vector Sequence |